Atomic structure of thymidylate synthase: Target for rational drug design

Larry W. Hardy, Janet S. Finer-Moore, William R. Montfort, Melvin O. Jones, Daniel V. Santi, Robert M. Stroud

Research output: Contribution to journalArticlepeer-review

316 Scopus citations


The atomic structure of thymidylate synthase from Lactobacillus casei was determined at 3 angstrom resolution. The native enzyme is a dimer of identical subunits. The dimer interface is formed by an unusual association between five-stranded β sheets present in each monomer. Comparison of known sequences with the Lactobacillus casei structure suggests that they all have a common core structure around which loops are inserted or deleted in different sequences. Residues from both subunits contribute to each active site. Two arginine side chains can contribute to binding phosphate on the substrate. The side chains of several conserved amino acids can account for other determinants of substrate binding.

Original languageEnglish (US)
Pages (from-to)448-455
Number of pages8
Issue number4787
StatePublished - 1987

ASJC Scopus subject areas

  • General


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