Asthma and endotoxin: Lipopolysaccharide-binding protein and soluble CD14 in bronchoalveolar compartment

Wendy Dubin, Thomas R. Martin, Peggy Swoveland, Didier J. Leturcq, Ann M. Moriarty, Peter S. Tobias, Eugene R. Bleecker, Simeon E. Goldblum, Jeffrey D. Hasday

Research output: Contribution to journalArticlepeer-review

124 Scopus citations


In allergic asthma, inhalation of antigen provokes an early increase in microvascular permeability with protein extravasation and a delayed recruitment of inflammatory cells. We showed that similar concentrations of lipopolysaccharide (LPS) are present in bronchoalveolar lavage fluid (BALF) in 12 subjects without asthma (86.5 ± 53.8 pg/ml) and 12 subjects with mild asthma (111 ± 37.0 pg/ml). These LPS levels are insufficient to stimulate cytokine release without accessory molecules. BALF obtained 24 h after segmental ragweed antigen challenge in 11 asthmatics allergic to ragweed contained increased levels of two LPS accessory molecules compared with preantigen BALF, 158-fold more LPS-binding protein (LBP) (4.83 ± 2.02 vs. 742 ± 387 ng/ml; P < 0.03) and 31.6-fold more soluble CD14 (sCD14) (3.45 ± 1.04 vs. 110 ± 51.6 ng/ml; P < 0.02). Postantigen BALF enhanced binding of fluorescein-conjugated LPS to CD14-bearing THP-1 cells and supported LPS- induced non-CD14-bearing endothelial cell expression of intercellular adhesion molecule-1 and interleukin-6, indicating functional LBP and sCD14. We suggest that extravasation of LBP and sCD14 into the bronchoalveolar compartment after antigen inhalation may enhance the capacity of inhaled or aspirated LPS to activate an inflammatory cascade that may amplify the inflammatory response to inhaled antigen in some asthmatics.

Original languageEnglish (US)
Pages (from-to)L736-L744
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Issue number5 14-5
StatePublished - May 1996
Externally publishedYes


  • bronchoalveolar lavage
  • endothelial cell
  • interleukin-6
  • macrophage
  • segmental allergen challenge

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology


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