Association of the bloom syndrome protein with topoisomerase IIIα in somatic and meiotic cells

F. Brad Johnson; David B. Lombard; Norma F. Neff; Mary Ann Mastrangelo; William Dewolf; Nathan A. Ellis; Robert A. Marciniak; Yizhong Yin; Rudolf Jaenisch; Leonard Guarente

Association of the bloom syndrome protein with topoisomerase IIIα in somatic and meiotic cells

F. Brad Johnson, David B. Lombard, Norma F. Neff, Mary Ann Mastrangelo, William Dewolf, Nathan A. Ellis, Robert A. Marciniak, Yizhong Yin, Rudolf Jaenisch, Leonard Guarente

Research output: Contribution to journal › Article › peer-review

149 Scopus citations

Abstract

Bloom syndrome (BS) is characterized by genomic instability and cancer susceptibility caused by defects in BLM, a DNA helicase of the RecQ-family (J. German and N.A. Ellis, The Genetic Basis of Human Cancer, pp. 301-316, 1998). RecQ helicases and topoisomerase III proteins interact physically and functionally in yeast (S. Gangloff et al., Mol. Cell. Biol., 14: 8391-8398, 1994) and in Escherichia coli can function together to enable passage of double-stranded DNA (F. G. Harmon et al., Mol. Cell, 3: 611-620, 1999). We demonstrate in somatic and meiotic human cells an association between BLM and topoisomerase IIIα. These proteins colocalize in promyelocytic leukemia protein nuclear bodies, and this localization is disrupted in BS cells. Thus, mechanisms by which RecQ helicases and topoisomerase III proteins cooperate to maintain genomic stability in model organisms likely apply to humans.

Original language	English (US)
Pages (from-to)	1162-1167
Number of pages	6
Journal	Cancer Research
Volume	60
Issue number	5
State	Published - Mar 1 2000
Externally published	Yes

ASJC Scopus subject areas

Oncology
Cancer Research

Cite this

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title = "Association of the bloom syndrome protein with topoisomerase IIIα in somatic and meiotic cells",

abstract = "Bloom syndrome (BS) is characterized by genomic instability and cancer susceptibility caused by defects in BLM, a DNA helicase of the RecQ-family (J. German and N.A. Ellis, The Genetic Basis of Human Cancer, pp. 301-316, 1998). RecQ helicases and topoisomerase III proteins interact physically and functionally in yeast (S. Gangloff et al., Mol. Cell. Biol., 14: 8391-8398, 1994) and in Escherichia coli can function together to enable passage of double-stranded DNA (F. G. Harmon et al., Mol. Cell, 3: 611-620, 1999). We demonstrate in somatic and meiotic human cells an association between BLM and topoisomerase IIIα. These proteins colocalize in promyelocytic leukemia protein nuclear bodies, and this localization is disrupted in BS cells. Thus, mechanisms by which RecQ helicases and topoisomerase III proteins cooperate to maintain genomic stability in model organisms likely apply to humans.",

author = "Johnson, {F. Brad} and Lombard, {David B.} and Neff, {Norma F.} and Mastrangelo, {Mary Ann} and William Dewolf and Ellis, {Nathan A.} and Marciniak, {Robert A.} and Yizhong Yin and Rudolf Jaenisch and Leonard Guarente",

year = "2000",

month = mar,

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language = "English (US)",

volume = "60",

pages = "1162--1167",

journal = "Cancer Research",

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TY - JOUR

T1 - Association of the bloom syndrome protein with topoisomerase IIIα in somatic and meiotic cells

AU - Johnson, F. Brad

AU - Lombard, David B.

AU - Neff, Norma F.

AU - Mastrangelo, Mary Ann

AU - Dewolf, William

AU - Ellis, Nathan A.

AU - Marciniak, Robert A.

AU - Yin, Yizhong

AU - Jaenisch, Rudolf

AU - Guarente, Leonard

PY - 2000/3/1

Y1 - 2000/3/1

N2 - Bloom syndrome (BS) is characterized by genomic instability and cancer susceptibility caused by defects in BLM, a DNA helicase of the RecQ-family (J. German and N.A. Ellis, The Genetic Basis of Human Cancer, pp. 301-316, 1998). RecQ helicases and topoisomerase III proteins interact physically and functionally in yeast (S. Gangloff et al., Mol. Cell. Biol., 14: 8391-8398, 1994) and in Escherichia coli can function together to enable passage of double-stranded DNA (F. G. Harmon et al., Mol. Cell, 3: 611-620, 1999). We demonstrate in somatic and meiotic human cells an association between BLM and topoisomerase IIIα. These proteins colocalize in promyelocytic leukemia protein nuclear bodies, and this localization is disrupted in BS cells. Thus, mechanisms by which RecQ helicases and topoisomerase III proteins cooperate to maintain genomic stability in model organisms likely apply to humans.

AB - Bloom syndrome (BS) is characterized by genomic instability and cancer susceptibility caused by defects in BLM, a DNA helicase of the RecQ-family (J. German and N.A. Ellis, The Genetic Basis of Human Cancer, pp. 301-316, 1998). RecQ helicases and topoisomerase III proteins interact physically and functionally in yeast (S. Gangloff et al., Mol. Cell. Biol., 14: 8391-8398, 1994) and in Escherichia coli can function together to enable passage of double-stranded DNA (F. G. Harmon et al., Mol. Cell, 3: 611-620, 1999). We demonstrate in somatic and meiotic human cells an association between BLM and topoisomerase IIIα. These proteins colocalize in promyelocytic leukemia protein nuclear bodies, and this localization is disrupted in BS cells. Thus, mechanisms by which RecQ helicases and topoisomerase III proteins cooperate to maintain genomic stability in model organisms likely apply to humans.

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SN - 0008-5472

VL - 60

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JO - Cancer Research

JF - Cancer Research

IS - 5

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Association of the bloom syndrome protein with topoisomerase IIIα in somatic and meiotic cells

Abstract

ASJC Scopus subject areas

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