Assessment of DNA yield and purity: An overlooked detail of PCR troubleshooting

Kelly A. Boesenberg-Smith; Mohammad M. Pessarakli; Donna M. Wolk

doi:10.1016/j.clinmicnews.2011.12.002

Assessment of DNA yield and purity: An overlooked detail of PCR troubleshooting

Kelly A. Boesenberg-Smith
, Mohammad M. Pessarakli
, Donna M. Wolk

Pathology and Lab Medicine

Research output: Contribution to journal › Article › peer-review

108 Scopus citations

Abstract

Determination of nucleic acid concentration and purity is important for downstream applications, such as polymerase chain reaction (PCR) and multiplex PCR. There are several methods for quantifying and characterizing DNA, and results describing concentration and purity vary by the extraction method, as well as by the DNA quantification method used. Understanding the methods used for quantification and purity measurements of DNA may aid laboratory scientists when troubleshooting PCR and help them to determine the best extraction method for each application.

Original language	English (US)
Pages (from-to)	1-6
Number of pages	6
Journal	Clinical Microbiology Newsletter
Volume	34
Issue number	1
DOIs	https://doi.org/10.1016/j.clinmicnews.2011.12.002
State	Published - Jan 1 2012

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

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10.1016/j.clinmicnews.2011.12.002

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AB - Determination of nucleic acid concentration and purity is important for downstream applications, such as polymerase chain reaction (PCR) and multiplex PCR. There are several methods for quantifying and characterizing DNA, and results describing concentration and purity vary by the extraction method, as well as by the DNA quantification method used. Understanding the methods used for quantification and purity measurements of DNA may aid laboratory scientists when troubleshooting PCR and help them to determine the best extraction method for each application.

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Assessment of DNA yield and purity: An overlooked detail of PCR troubleshooting

Abstract

ASJC Scopus subject areas

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