Assessment of constitutive activity in E-Type prostanoid receptors

Hiromichi Fujino, Toshihiko Murayama, John W. Regan

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

The potential for G-protein-coupled receptors (GPCRs) to show constitutive activity is emerging as one of the fundamental properties of GPCRs signal transduction. Indeed, of the four subtypes of E-type prostanoid (EP) receptors, the EP3 and EP4 subtypes show constitutive activity in addition to their innate ligand-dependent activation of signaling pathways. The constitutive activity of the EP3 and EP4 receptor subtypes was discovered during the initial characterizations of these receptors and may be important for setting the basal level of cellular tone in the given signaling pathway. This chapter introduces some of the methods that can be used to study the constitutive activity of the EP receptors.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages95-107
Number of pages13
EditionC
DOIs
StatePublished - 2010

Publication series

NameMethods in Enzymology
NumberC
Volume484
ISSN (Print)0076-6879

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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