TY - JOUR
T1 - Assessment of cell-specific cytotoxic responses of the kidney to selected aromatic hydrocarbons
AU - Bowes, R. C.
AU - Ramos, K. S.
N1 - Funding Information:
Acknowledgements--The authors thank S. H. Safe for kindly providing TCDD and TCDF. K.S.R. is a recipient of Research Career Development Award (ES 00213) from the National Institute of Environmental Health Sciences. R. C. B. III is a pre-doctoral fellow in the NIEHS Toxicology Training Grant (ES 07273-02). We would also like to thank Estelle Hejtmancik and Eva Binford for their assistance with the potassium content assay. This work was supported in part by NIEHS Superfund Program Project Grant 04917 to K.S.R.
PY - 1994/12
Y1 - 1994/12
N2 - The present studies were conducted to assess cell-specific cytotoxic responses of the kidney to selected aromatic hydrocarbons (AHs) using in vitro culture systems of glomerular mesangial cells and cortical tubular epithelial cells. Primary cultures of glomerular mesangial cells or cortical tubular epithelial cells were treated for 24 hr with naphthalene (NAPH, 0.1-1000 μm), 2-methylnaphthalene (2-MNAPH, 0.1-1000 μm), benzo[a]pyrene (B[a]P, 0.3-300 μm), pentachlorophenol (PCP, 0.1-1000 μm), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, 0.001-1 nm) or 2,3,7,8-tetrachlorodibenzofuran (TCDF, 0.005-5 nm). [3H]Thymidine incorporation into DNA, cellular potassium content, and lactate dehydrogenase (LDH) leakage were used as indices of cytotoxicity. Glomerular mesangial cells exposed to NAPH or 2-MNAPH (0.1-1000 μm) exhibited [3H]thymidine incorporation profiles similar to those of the vehicle controls, whereas B[a]P at all concentrations and PCP at 1000 μm decreased [3H]thymidine incorporation in comparison with the controls. TCDD and TCDF increased [3H]thymidine incorporation relative to controls at 0.1 nm and 0.5 nm, respectively. Glomerular mesangial cells treated with NAPH or PCP exhibited decreased cellular potassium content and increased LDH leakage only at the 1000 μm concentration. In tubular epithelial cells, treatment with NAPH (10 or 1000 μm), 2-MNAPH (10 or 1000 μm), B[a]P (300 μm), and PCP (10 or 1000 μm) induced concentration-dependent decreases in [3H]thymidine incorporation relative to controls. In contrast, exposure of tubular epithelial cells to TCDD or TCDF did not modulate [3H]thymidine incorporation at any of the concentrations tested. A modest decrease in cellular potassium content and an increase in LDH leakage was observed at the 1000 μm concentration of PCP in tubular epithelial cells. On the basis of these data, we conclude that renal glomerular mesangial cells and tubular epithelial cells exhibit differential cytotoxic response profiles to AHs.
AB - The present studies were conducted to assess cell-specific cytotoxic responses of the kidney to selected aromatic hydrocarbons (AHs) using in vitro culture systems of glomerular mesangial cells and cortical tubular epithelial cells. Primary cultures of glomerular mesangial cells or cortical tubular epithelial cells were treated for 24 hr with naphthalene (NAPH, 0.1-1000 μm), 2-methylnaphthalene (2-MNAPH, 0.1-1000 μm), benzo[a]pyrene (B[a]P, 0.3-300 μm), pentachlorophenol (PCP, 0.1-1000 μm), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, 0.001-1 nm) or 2,3,7,8-tetrachlorodibenzofuran (TCDF, 0.005-5 nm). [3H]Thymidine incorporation into DNA, cellular potassium content, and lactate dehydrogenase (LDH) leakage were used as indices of cytotoxicity. Glomerular mesangial cells exposed to NAPH or 2-MNAPH (0.1-1000 μm) exhibited [3H]thymidine incorporation profiles similar to those of the vehicle controls, whereas B[a]P at all concentrations and PCP at 1000 μm decreased [3H]thymidine incorporation in comparison with the controls. TCDD and TCDF increased [3H]thymidine incorporation relative to controls at 0.1 nm and 0.5 nm, respectively. Glomerular mesangial cells treated with NAPH or PCP exhibited decreased cellular potassium content and increased LDH leakage only at the 1000 μm concentration. In tubular epithelial cells, treatment with NAPH (10 or 1000 μm), 2-MNAPH (10 or 1000 μm), B[a]P (300 μm), and PCP (10 or 1000 μm) induced concentration-dependent decreases in [3H]thymidine incorporation relative to controls. In contrast, exposure of tubular epithelial cells to TCDD or TCDF did not modulate [3H]thymidine incorporation at any of the concentrations tested. A modest decrease in cellular potassium content and an increase in LDH leakage was observed at the 1000 μm concentration of PCP in tubular epithelial cells. On the basis of these data, we conclude that renal glomerular mesangial cells and tubular epithelial cells exhibit differential cytotoxic response profiles to AHs.
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U2 - 10.1016/0887-2333(94)90104-X
DO - 10.1016/0887-2333(94)90104-X
M3 - Article
AN - SCOPUS:0028090488
SN - 0887-2333
VL - 8
SP - 1151
EP - 1160
JO - Toxicology in Vitro
JF - Toxicology in Vitro
IS - 6
ER -