Assessing protein oxidation by inorganic nanoparticles with enzyme-linked immunosorbent assay (ELISA)

Wenjie Sun, Antonia Luna-Velasco, Reyes Sierra-Alvarez, Jim A. Field

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Growth in the nanotechnology industry is leading to increased production of engineered nanoparticles (NPs). This has given rise to concerns about the potential adverse and toxic effects to biological system and the environment. An important mechanism of NP toxicity is oxidative stress caused by the formation of reactive oxygen species (ROS) or via direct oxidation of biomolecules. In this study, a protein oxidation assay was developed as an indicator of biomolecule oxidation by NPs. The oxidation of the protein, bovine serum albumin (BSA) was evaluated with an enzyme-linked immunosorbent assay (ELISA) to measure the protein carbonyl derivatives formed from protein oxidation. The results showed that some NPs such as Cu(0), CuO, Mn2O3, and Fe(0) caused oxidation of BSA; whereas, many of the other NPs tested were not reactive or very slowly reactive with BSA. The mechanisms involved in the oxidation of BSA protein by the reactive NPs could be attributed to the combined effects of ROS-dependent and direct protein oxidation mechanisms. The ELISA assay is a promising method for the assessment of protein oxidation by NPs, which can provide insights on NP toxicity mechanisms.

Original languageEnglish (US)
Pages (from-to)694-701
Number of pages8
JournalBiotechnology and Bioengineering
Issue number3
StatePublished - Mar 2013


  • Inorganic nanoparticles
  • Nanotoxicity
  • Protein oxidation
  • Reactive oxygen species (ROS)

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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