Assembly of Model Membrane Nanodiscs for Native Mass Spectrometry

Marius M. Kostelic, Ciara K. Zak, Hiruni S. Jayasekera, Michael T. Marty

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Native mass spectrometry (MS) with nanodiscs is a promising technique for characterizing membrane protein and peptide interactions in lipid bilayers. However, prior studies have used nanodiscs made of only one or two lipids, which lack the complexity of a natural lipid bilayer. To better model specific biological membranes, we developed model mammalian, bacterial, and mitochondrial nanodiscs with up to four different phospholipids. Careful selection of lipids with similar masses that balance the fluidity and curvature enabled these complex nanodiscs to be assembled and resolved with native MS. We then applied this approach to characterize the specificity and incorporation of LL-37, a human antimicrobial peptide, in single-lipid nanodiscs versus model bacterial nanodiscs. Overall, development of these model membrane nanodiscs reveals new insights into the assembly of complex nanodiscs and provides a useful toolkit for studying membrane protein, peptide, and lipid interactions in model biological membranes.

Original languageEnglish (US)
Pages (from-to)5972-5979
Number of pages8
JournalAnalytical Chemistry
Volume93
Issue number14
DOIs
StatePublished - Apr 13 2021

ASJC Scopus subject areas

  • Analytical Chemistry

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