Ascorbic acid promotes recovery of cellular functions following toxicant-induced injury

Grazyna Nowak, Charleata A. Carter, Rick G. Schnellmann

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

We have shown that renal proximal tubular cells (RPTC) recover cellular functions following sublethal injury induced by the oxidant t-butylhydroperoxide but not by the nephrotoxic cysteine conjugate dichlorovinyl-L-cysteine (DCVC). This study investigated whether L-ascorbic acid phosphate (AscP) promotes recovery of RPTC functions following DCVC-induced injury. DCVC exposure (200 μM; 100 min) resulted in 60% RPTC death and loss from the monolayer at 24 h independent of physiological (50 μM) or pharmacological (500 μM) AscP concentrations. Likewise, the DCVC-induced decrease in mitochondrial function (54%), active Na+ transport (66%), and Na+-K+-ATPase activity (77%) was independent of the AscP concentration. Analysis of Na+-K+-ATPase protein expression and distribution in the plasma membrane using immunocytochemistry and confocal laser scanning microscopy revealed the loss of Na+-K+-ATPase protein from the basolateral membrane of RPTC treated with DCVC. DCVC-injured RPTC cultured in the presence of 50 μM AscP did not proliferate nor recover their physiological functions over time. In contrast, RPTC cultured in the presence of 500 μM AscP proliferated, recovered all examined physiological functions, and the basolateral membrane expression of Na+-K+-ATPase by day 4 following DCVC injury. These results demonstrate that pharmacological concentrations of AscP do not prevent toxicant-induced cell injury and death but promote complete recovery of mitochondrial function, active Na+ transport, and proliferation following toxicant-induced injury. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)37-45
Number of pages9
JournalToxicology and Applied Pharmacology
Volume167
Issue number1
DOIs
StatePublished - Aug 15 2000
Externally publishedYes

Keywords

  • Active sodium transport
  • Ascorbic acid
  • Cell repair
  • Cysteine conjugate
  • DCVC
  • Mitochondrial function
  • Oxygen consumption
  • Proliferation
  • Regeneration
  • Renal proximal tubular cells
  • S-(1, 2-dichlorovinyl)-L-cysteine
  • Sodium-potassium adenosinetriphosphatase
  • Sublethal cell injury

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

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