Applications of a colorimetric plate assay for soluble methane monooxygenase activity

D. W. Graham; D. G. Korich; R. P. LeBlanc; N. A. Sinclair; R. G. Arnold

doi:10.1128/aem.58.7.2231-2236.1992

Applications of a colorimetric plate assay for soluble methane monooxygenase activity

D. W. Graham, D. G. Korich, R. P. LeBlanc, N. A. Sinclair, R. G. Arnold

Chemical and Environmental Engineering

Research output: Contribution to journal › Article › peer-review

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Abstract

A straightforward method is described for screening methanotrophic colonies for soluble methane monooxygenase (sMMO) activity on solid media. Such activity results in the development of a colored complex between 1- naphthol, which is formed when sMMO reacts with naphthalene, and o- dianisidine (tetrazotized). Methanotrophic colonies expressing sMMO turned deep purple when exposed successively to naphthalene and o-dianisidine. The method was evaluated within the contexts of two potential applications. The first was for the enumeration of Methylosinus trichosporium OB3b in a methane-amended, unsaturated soil column dedicated to vinyl chloride treatment. The second application was for the isolation and enumeration of sMMO-bearing methanotrophs from sanitary landfill soils. The technique was effective in both applications.

Original language	English (US)
Pages (from-to)	2231-2236
Number of pages	6
Journal	Applied and environmental microbiology
Volume	58
Issue number	7
DOIs	https://doi.org/10.1128/aem.58.7.2231-2236.1992
State	Published - 1992

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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10.1128/aem.58.7.2231-2236.1992

Cite this

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title = "Applications of a colorimetric plate assay for soluble methane monooxygenase activity",

abstract = "A straightforward method is described for screening methanotrophic colonies for soluble methane monooxygenase (sMMO) activity on solid media. Such activity results in the development of a colored complex between 1- naphthol, which is formed when sMMO reacts with naphthalene, and o- dianisidine (tetrazotized). Methanotrophic colonies expressing sMMO turned deep purple when exposed successively to naphthalene and o-dianisidine. The method was evaluated within the contexts of two potential applications. The first was for the enumeration of Methylosinus trichosporium OB3b in a methane-amended, unsaturated soil column dedicated to vinyl chloride treatment. The second application was for the isolation and enumeration of sMMO-bearing methanotrophs from sanitary landfill soils. The technique was effective in both applications.",

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AU - Graham, D. W.

AU - Korich, D. G.

AU - LeBlanc, R. P.

AU - Sinclair, N. A.

AU - Arnold, R. G.

PY - 1992

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AB - A straightforward method is described for screening methanotrophic colonies for soluble methane monooxygenase (sMMO) activity on solid media. Such activity results in the development of a colored complex between 1- naphthol, which is formed when sMMO reacts with naphthalene, and o- dianisidine (tetrazotized). Methanotrophic colonies expressing sMMO turned deep purple when exposed successively to naphthalene and o-dianisidine. The method was evaluated within the contexts of two potential applications. The first was for the enumeration of Methylosinus trichosporium OB3b in a methane-amended, unsaturated soil column dedicated to vinyl chloride treatment. The second application was for the isolation and enumeration of sMMO-bearing methanotrophs from sanitary landfill soils. The technique was effective in both applications.

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Applications of a colorimetric plate assay for soluble methane monooxygenase activity

Abstract

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