APPLICATION OF GENE PROBES TO POLIOVIRUS AND HEPATITIS A VIRUS DETECTION IN WATER AND SHELLFISH.

Aaron B. Margolin; Michael S. Bitrick; Ricardo DeLeon; Charles P. Gerba

doi:10.1109/oceans.1987.1160617

APPLICATION OF GENE PROBES TO POLIOVIRUS AND HEPATITIS A VIRUS DETECTION IN WATER AND SHELLFISH.

Aaron B. Margolin, Michael S. Bitrick, Ricardo DeLeon, Charles P. Gerba

Research output: Contribution to journal › Conference article › peer-review

1 Scopus citations

Abstract

The authors have developed cDNA probes for poliovirus type 1 and hepatitis A virus labeled with both **3**2P dATP and dCTP which are reported to be capable of detecting as little as one femtogram of nucleic acid, one tissue culture infectious dose of poliovirus type 1 or one radio-immuno-focus unit of hepatitis A virus. Results are obtained within 72 hours as cell culture is not necessary. The authors have successfully applied this method to the detection of enteroviruses in both water and shellfish concentrates.

Original language	English (US)
Pages (from-to)	1746-1751
Number of pages	6
Journal	Oceans Conference Record (IEEE)
DOIs	https://doi.org/10.1109/oceans.1987.1160617
State	Published - 1987

ASJC Scopus subject areas

Oceanography
Ocean Engineering

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10.1109/oceans.1987.1160617

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title = "APPLICATION OF GENE PROBES TO POLIOVIRUS AND HEPATITIS A VIRUS DETECTION IN WATER AND SHELLFISH.",

abstract = "The authors have developed cDNA probes for poliovirus type 1 and hepatitis A virus labeled with both **3**2P dATP and dCTP which are reported to be capable of detecting as little as one femtogram of nucleic acid, one tissue culture infectious dose of poliovirus type 1 or one radio-immuno-focus unit of hepatitis A virus. Results are obtained within 72 hours as cell culture is not necessary. The authors have successfully applied this method to the detection of enteroviruses in both water and shellfish concentrates.",

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year = "1987",

doi = "10.1109/oceans.1987.1160617",

language = "English (US)",

pages = "1746--1751",

journal = "Oceans Conference Record (IEEE)",

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publisher = "Institute of Electrical and Electronics Engineers Inc.",

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AU - Margolin, Aaron B.

AU - Bitrick, Michael S.

AU - DeLeon, Ricardo

AU - Gerba, Charles P.

PY - 1987

Y1 - 1987

N2 - The authors have developed cDNA probes for poliovirus type 1 and hepatitis A virus labeled with both **3**2P dATP and dCTP which are reported to be capable of detecting as little as one femtogram of nucleic acid, one tissue culture infectious dose of poliovirus type 1 or one radio-immuno-focus unit of hepatitis A virus. Results are obtained within 72 hours as cell culture is not necessary. The authors have successfully applied this method to the detection of enteroviruses in both water and shellfish concentrates.

AB - The authors have developed cDNA probes for poliovirus type 1 and hepatitis A virus labeled with both **3**2P dATP and dCTP which are reported to be capable of detecting as little as one femtogram of nucleic acid, one tissue culture infectious dose of poliovirus type 1 or one radio-immuno-focus unit of hepatitis A virus. Results are obtained within 72 hours as cell culture is not necessary. The authors have successfully applied this method to the detection of enteroviruses in both water and shellfish concentrates.

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DO - 10.1109/oceans.1987.1160617

M3 - Conference article

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JO - Oceans Conference Record (IEEE)

JF - Oceans Conference Record (IEEE)

ER -

APPLICATION OF GENE PROBES TO POLIOVIRUS AND HEPATITIS A VIRUS DETECTION IN WATER AND SHELLFISH.

Abstract

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