Apoptosis does not contribute to the blood lymphocytopenia observed after intensive and downhill treadmill running in humans

Richard J. Simpson, Geraint D. Florida-James, Greg P. Whyte, James R. Black, James A. Ross, Keith Guy

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

The lymphocytopenia that occurs during the recovery stage of exercise may be a result of apoptosis through an increased expression of CD95, a loss of the complement regulatory proteins CD55 and CD59, or both. Trained subjects completed intensive, moderate, and downhill treadmill-running protocols. Blood lymphocytes isolated before, immediately after, 1h after, and 24h after each exercise test were assessed for markers of apoptosis (Annexin-V+, HSP60+), and CD55, CD59, and CD95 expression by flow cytometry. Lymphocytopenia occurred 1h after intensive and downhill running exercise, but no changes in the percentage of Annexin-V + or HSP60 + lymphocytes were found. Numbers of CD95+, CD55dim, and CD59dim lymphocytes increased immediately after intensive and downhill exercise, which were attributed to the selective mobilization and subsequent efflux of CD8+ and CD56+ lymphocyte subsets. No differences were found between the intensive and downhill protocols. In conclusion, apoptosis of circulating lymphocytes does not appear to contribute to exercise-induced lymphocytopenia.

Original languageEnglish (US)
Pages (from-to)157-174
Number of pages18
JournalResearch in Sports Medicine
Volume15
Issue number3
DOIs
StatePublished - Jul 2007
Externally publishedYes

Keywords

  • CD antigens
  • Complement regulatory proteins
  • Flow cytometry
  • Heat-shock protein-60
  • Lymphocytosis
  • Muscle damage

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Fingerprint

Dive into the research topics of 'Apoptosis does not contribute to the blood lymphocytopenia observed after intensive and downhill treadmill running in humans'. Together they form a unique fingerprint.

Cite this