TY - JOUR
T1 - Antigenic relationships of murine coronaviruses
T2 - Analysis using monoclonal antibodies to JHM (MHV-4) virus
AU - Fleming, John O.
AU - Stohlman, Stephen A.
AU - Harmon, Richard C.
AU - McLai, Michael
AU - Frelinger, Jeffrey A.
AU - Weiner, Leslie P.
N1 - Funding Information:
We thank Chris Patton, Natalie Stein, Todd Kennell, and Gabriele Olivka for excellent technical assistance and Raymond Mitchell, Alisa Young, and Claudia Troesch for editorial assistance with the manuscript. Alicia McDonough and Scott Linthicum generously supplied osI-labeled protein A, and Minnie McMillan kindly assisted in the preparation of figures. This work was supported by grants from the National Institutes of Health, Grants CA 22662 and NS 18146, the Multiple Sclerosis Society, Grant 1449 Al, and the National Science Foundation, Grant PCM 10372 . R . Harmon is a recipient of National Research Service Award NS-07149, and J . Frelinger is a recipient of an American Cancer Society Faculty Research Award .
PY - 1983/12
Y1 - 1983/12
N2 - Monoclonal antibodies were produced to JHMV-DL, a neurotropic member of the mouse hepatitis virus (MHV) or murine coronavirus group. Of 23 antibodies isolated, 10 were specific for the major envelope glycoprotein, gp180/90, 10 for the nucleocapsid protein, pp60, and 3 for the minor envelope glycoprotein, gp25. Eleven different MHV isolates were used in antibody binding assays to study antigenic relationships among the viruses. Each MHV isolate tested had a unique pattern of antibody binding, indicating that each is a distinct strain. Conservation of JHMV-DL antigenic determinants varied among the three proteins, with pp60 showing intermediate conservation, gp180/90 little conservation, and gp25 marked conservation in the different MHV strains. Monoclonal antibodies to pp60 proved most useful in delineating antigenic relationships among MHV strains. These antigenic groups correlated with pathogenic types, indicating that pp60 may be one of the gene products which mediates the distinct disease patterns manifested by different murine coronaviruses.
AB - Monoclonal antibodies were produced to JHMV-DL, a neurotropic member of the mouse hepatitis virus (MHV) or murine coronavirus group. Of 23 antibodies isolated, 10 were specific for the major envelope glycoprotein, gp180/90, 10 for the nucleocapsid protein, pp60, and 3 for the minor envelope glycoprotein, gp25. Eleven different MHV isolates were used in antibody binding assays to study antigenic relationships among the viruses. Each MHV isolate tested had a unique pattern of antibody binding, indicating that each is a distinct strain. Conservation of JHMV-DL antigenic determinants varied among the three proteins, with pp60 showing intermediate conservation, gp180/90 little conservation, and gp25 marked conservation in the different MHV strains. Monoclonal antibodies to pp60 proved most useful in delineating antigenic relationships among MHV strains. These antigenic groups correlated with pathogenic types, indicating that pp60 may be one of the gene products which mediates the distinct disease patterns manifested by different murine coronaviruses.
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U2 - 10.1016/0042-6822(83)90498-1
DO - 10.1016/0042-6822(83)90498-1
M3 - Article
C2 - 6318433
AN - SCOPUS:0021080239
SN - 0042-6822
VL - 131
SP - 296
EP - 307
JO - Virology
JF - Virology
IS - 2
ER -