TY - JOUR
T1 - Antigenic differences within the Cryptosporidium hominis and Cryptosporidium parvum surface proteins P23 and GP900 defined by monoclonal antibody reactivity
AU - Sturbaum, Gregory D.
AU - Schaefer, Deborah A.
AU - Jost, B. Helen
AU - Sterling, Charles R.
AU - Riggs, Michael W.
N1 - Funding Information:
The authors thank Walter Narro for purifying the isolates used in this study. We also thank Drs. Christina M. Coughlan and Stephen J. Billington for scientific discussions and review of the manuscript. This work was supported in part by Grant Number AI 056944 from the National Institute of Allergy and Infectious Disease.
PY - 2008/6
Y1 - 2008/6
N2 - The biological basis for the specificity of host infectivity patterns of Cryptosporidium spp., in particular C. hominis and C. parvum, has yet to be fully elucidated. Comparison of the C. parvum and C. hominis P23 and GP900 predicted amino acid sequences revealed 3 differences in P23 and 4 and 17 differences in GP900 domains 1 and 5, respectively. Using monoclonal antibodies developed against the surface (glyco)proteins P23 and GP900 of the C. parvum Iowa isolate, solubilized glycoprotein from three C. hominis isolates was screened for reactivity using Western immunoblots. One of ten P23 MAbs and three of 21 GP900 MAbs were not reactive with any of the three C. hominis isolates. The non-reactive P23 MAb binds to a peptide epitope, while the non-reactive GP900 MAbs bind to either carbohydrate/carbohydrate-dependent or peptide epitopes of C. parvum. These results demonstrate phenotypic differences between C. hominis and C. parvum within two (glyco)proteins that are involved in parasite gliding motility and attachment/invasion.
AB - The biological basis for the specificity of host infectivity patterns of Cryptosporidium spp., in particular C. hominis and C. parvum, has yet to be fully elucidated. Comparison of the C. parvum and C. hominis P23 and GP900 predicted amino acid sequences revealed 3 differences in P23 and 4 and 17 differences in GP900 domains 1 and 5, respectively. Using monoclonal antibodies developed against the surface (glyco)proteins P23 and GP900 of the C. parvum Iowa isolate, solubilized glycoprotein from three C. hominis isolates was screened for reactivity using Western immunoblots. One of ten P23 MAbs and three of 21 GP900 MAbs were not reactive with any of the three C. hominis isolates. The non-reactive P23 MAb binds to a peptide epitope, while the non-reactive GP900 MAbs bind to either carbohydrate/carbohydrate-dependent or peptide epitopes of C. parvum. These results demonstrate phenotypic differences between C. hominis and C. parvum within two (glyco)proteins that are involved in parasite gliding motility and attachment/invasion.
KW - Cryptosporidium hominis
KW - Cryptosporidium parvum
KW - Polymorphism
KW - Surface glycoprotein
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U2 - 10.1016/j.molbiopara.2008.02.009
DO - 10.1016/j.molbiopara.2008.02.009
M3 - Article
C2 - 18400317
AN - SCOPUS:42949164919
SN - 0166-6851
VL - 159
SP - 138
EP - 141
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 2
ER -