Anti-EGFR antibody conjugated organic-inorganic hybrid lipid nanovesicles selectively target tumor cells

Siu Ling Leung; Zhengbao Zha; Celine Cohn; Zhifei Dai; Xiaoyi Wu

doi:10.1016/j.colsurfb.2014.06.011

Anti-EGFR antibody conjugated organic-inorganic hybrid lipid nanovesicles selectively target tumor cells

Siu Ling Leung, Zhengbao Zha, Celine Cohn, Zhifei Dai, Xiaoyi Wu

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Chemical conjugation of anti-epidermal growth factor receptor monoclonal antibodies (anti-EGFR mAbs) to organic-inorganic hybrid liposomal immunocerasomes via maleimide-thiol coupling chemistry is explored as a mechanism for selectively targeting cancer cells. The cellular uptake and internalization of immunocerasomes are investigated in A431 cells that express an abnormally high level of EGFR, DU145 cells that overexpress EGFR, and HL-60 cells that are used as a negative control. The internalization study reveals a strong correlation between the receptor-mediated endocytosis of immunocerasomes and the membrane expression of EGFR. Further, free anti-EGFR mAbs and immunocerasomes conjugated with anti-EGFR mAbs at nanomolar doses display similar anti-proliferative effects on A431 cells. Additionally, serum proteins greatly reduce the cellular uptake of cerasomes that is mediated by non-specific receptors, but have no adverse effects on the specific EGFR-mediated delivery of immunocerasomes to A431 cells.

Original language	English (US)
Pages (from-to)	141-149
Number of pages	9
Journal	Colloids and Surfaces B: Biointerfaces
Volume	121
DOIs	https://doi.org/10.1016/j.colsurfb.2014.06.011
State	Published - Sep 1 2014

Keywords

Anti-EGFR antibody
Cancer cells
Organic-inorganic hybrid lipid nanovesicles
Selective targeting

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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10.1016/j.colsurfb.2014.06.011

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title = "Anti-EGFR antibody conjugated organic-inorganic hybrid lipid nanovesicles selectively target tumor cells",

abstract = "Chemical conjugation of anti-epidermal growth factor receptor monoclonal antibodies (anti-EGFR mAbs) to organic-inorganic hybrid liposomal immunocerasomes via maleimide-thiol coupling chemistry is explored as a mechanism for selectively targeting cancer cells. The cellular uptake and internalization of immunocerasomes are investigated in A431 cells that express an abnormally high level of EGFR, DU145 cells that overexpress EGFR, and HL-60 cells that are used as a negative control. The internalization study reveals a strong correlation between the receptor-mediated endocytosis of immunocerasomes and the membrane expression of EGFR. Further, free anti-EGFR mAbs and immunocerasomes conjugated with anti-EGFR mAbs at nanomolar doses display similar anti-proliferative effects on A431 cells. Additionally, serum proteins greatly reduce the cellular uptake of cerasomes that is mediated by non-specific receptors, but have no adverse effects on the specific EGFR-mediated delivery of immunocerasomes to A431 cells.",

keywords = "Anti-EGFR antibody, Cancer cells, Organic-inorganic hybrid lipid nanovesicles, Selective targeting",

author = "Leung, {Siu Ling} and Zhengbao Zha and Celine Cohn and Zhifei Dai and Xiaoyi Wu",

note = "Funding Information: This work was supported by the US National Institutes of Health ( R21EB009160 ) and National Science Foundation ( CMMI0855890 ), and National Natural Science Foundation of China ( 2007AA03Z316 , 30970829 ). ",

year = "2014",

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doi = "10.1016/j.colsurfb.2014.06.011",

language = "English (US)",

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AU - Leung, Siu Ling

AU - Zha, Zhengbao

AU - Cohn, Celine

AU - Dai, Zhifei

AU - Wu, Xiaoyi

N1 - Funding Information: This work was supported by the US National Institutes of Health ( R21EB009160 ) and National Science Foundation ( CMMI0855890 ), and National Natural Science Foundation of China ( 2007AA03Z316 , 30970829 ).

PY - 2014/9/1

Y1 - 2014/9/1

N2 - Chemical conjugation of anti-epidermal growth factor receptor monoclonal antibodies (anti-EGFR mAbs) to organic-inorganic hybrid liposomal immunocerasomes via maleimide-thiol coupling chemistry is explored as a mechanism for selectively targeting cancer cells. The cellular uptake and internalization of immunocerasomes are investigated in A431 cells that express an abnormally high level of EGFR, DU145 cells that overexpress EGFR, and HL-60 cells that are used as a negative control. The internalization study reveals a strong correlation between the receptor-mediated endocytosis of immunocerasomes and the membrane expression of EGFR. Further, free anti-EGFR mAbs and immunocerasomes conjugated with anti-EGFR mAbs at nanomolar doses display similar anti-proliferative effects on A431 cells. Additionally, serum proteins greatly reduce the cellular uptake of cerasomes that is mediated by non-specific receptors, but have no adverse effects on the specific EGFR-mediated delivery of immunocerasomes to A431 cells.

AB - Chemical conjugation of anti-epidermal growth factor receptor monoclonal antibodies (anti-EGFR mAbs) to organic-inorganic hybrid liposomal immunocerasomes via maleimide-thiol coupling chemistry is explored as a mechanism for selectively targeting cancer cells. The cellular uptake and internalization of immunocerasomes are investigated in A431 cells that express an abnormally high level of EGFR, DU145 cells that overexpress EGFR, and HL-60 cells that are used as a negative control. The internalization study reveals a strong correlation between the receptor-mediated endocytosis of immunocerasomes and the membrane expression of EGFR. Further, free anti-EGFR mAbs and immunocerasomes conjugated with anti-EGFR mAbs at nanomolar doses display similar anti-proliferative effects on A431 cells. Additionally, serum proteins greatly reduce the cellular uptake of cerasomes that is mediated by non-specific receptors, but have no adverse effects on the specific EGFR-mediated delivery of immunocerasomes to A431 cells.

KW - Anti-EGFR antibody

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KW - Selective targeting

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Anti-EGFR antibody conjugated organic-inorganic hybrid lipid nanovesicles selectively target tumor cells

Abstract

Keywords

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