TY - JOUR
T1 - Angular Photodiode Array-Based Device to Detect Bacterial Pathogens in a Wound Model
AU - Sweeney, Robin E.
AU - Yoon, Jeong Yeol
N1 - Funding Information:
Manuscript received July 6, 2017; revised September 8, 2017; accepted September 11, 2017. Date of publication September 14, 2017; date of current version October 11, 2017. This work was supported by the Cardiovascular Biomedical Engineering Training Grant through the U.S. National Institutes of Health under Grant T32HL007955. This paper was presented at the SPIE Defense + Commercial Sensing Conference, April 2017, Anaheim, CA, USA (Proc. SPIE 10215: 102150U) and filed as a U.S. patent application number 62/320,093. The associate editor coordinating the review of this paper and approving it for publication was Dr. Ioannis Raptis. (Corresponding author: Jeong-Yeol Yoon.) The authors are with the Department of Biomedical Engineering, University of Arizona, Tucson, AZ 85721 USA (email: robinsweeney@ email.arizona.edu; [email protected]). Digital Object Identifier 10.1109/JSEN.2017.2752155
Publisher Copyright:
© 2017 IEEE.
PY - 2017/11/1
Y1 - 2017/11/1
N2 - We have developed a device that is able to rapidly and specifically diagnose bacterial pathogens in a wound model based on Mie scatter spectra from a tissue surface. The Mie scatter spectra collected is defined as the intensity of Mie scatter over the angle of detection from a tissue surface. A 650-nm LED perpendicular to the surface illuminates a tissue sample (90°) and photodiodes positioned in 10° increments from 10° to 80° of backscatter act as the detectors to collect these Mie scatter spectra. Through principal component analysis of the Mie scatter spectra collected, we have shown significant differences between Mie scatter spectra of tissues with bacterial pathogens versus those without, as well as significant differences between each species of bacteria tested. The device developed has been tested with a porcine dermis wound model, with samples inoculated with one of three bacterial species (Staphylococcus aureus, Escherichia coli, or Salmonella Typhimurium). Such a device could be critical in the monitoring of a wound for infection and rapid, specific diagnosis of a bacterial wound infection, which would significantly reduce the time and cost associated with specific diagnosis of a bacterial wound infection currently.
AB - We have developed a device that is able to rapidly and specifically diagnose bacterial pathogens in a wound model based on Mie scatter spectra from a tissue surface. The Mie scatter spectra collected is defined as the intensity of Mie scatter over the angle of detection from a tissue surface. A 650-nm LED perpendicular to the surface illuminates a tissue sample (90°) and photodiodes positioned in 10° increments from 10° to 80° of backscatter act as the detectors to collect these Mie scatter spectra. Through principal component analysis of the Mie scatter spectra collected, we have shown significant differences between Mie scatter spectra of tissues with bacterial pathogens versus those without, as well as significant differences between each species of bacteria tested. The device developed has been tested with a porcine dermis wound model, with samples inoculated with one of three bacterial species (Staphylococcus aureus, Escherichia coli, or Salmonella Typhimurium). Such a device could be critical in the monitoring of a wound for infection and rapid, specific diagnosis of a bacterial wound infection, which would significantly reduce the time and cost associated with specific diagnosis of a bacterial wound infection currently.
KW - Escherichia coli
KW - Mie scatter
KW - Staphylococcus aureus
KW - wound infection
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U2 - 10.1109/JSEN.2017.2752155
DO - 10.1109/JSEN.2017.2752155
M3 - Article
AN - SCOPUS:85030235823
SN - 1530-437X
VL - 17
SP - 6911
EP - 6917
JO - IEEE Sensors Journal
JF - IEEE Sensors Journal
IS - 21
M1 - 8038009
ER -