Angular anisotropy of 2H NMR spectral densities in phospholipid bilayers containing cholesterol

Theodore P. Trouard; Todd M. Alam; Jaroslav Zajicek; Michael F. Brown

doi:10.1016/0009-2614(92)85154-3

Angular anisotropy of ²H NMR spectral densities in phospholipid bilayers containing cholesterol

Theodore P. Trouard
, Todd M. Alam
, Jaroslav Zajicek
, Michael F. Brown

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Spin-lattice (R_1Z) and quadrupolar order (R_1Q) relaxation rates were measured for bilayers of macroscopically oriented 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC-d₅₄) containing cholesterol (1:1 molar ratio), using inversion recovery and broadband Jeener-Broekaert pulse sequences, respectively. Anisotropic deuterium (²H) NMR spin relaxation data were obtained for the first time along the entire flexible acyl chains of the phospholipid molecules in the liquid-crystalline state. Individual spectral densities J₁(ω₀) and J₂(2ω₀) were calculated from these relaxation rates, and a strong dependence on the angle θ between the macroscopic bilayer normal and the static magnetic field was observed. The spectral densities exhibited opposite angular anisotropies, which were explained in terms of a simple rotational diffusion model for the molecular dynamics of membrane lipid constituents.

Original language	English (US)
Pages (from-to)	67-75
Number of pages	9
Journal	Chemical Physics Letters
Volume	189
Issue number	1
DOIs	https://doi.org/10.1016/0009-2614(92)85154-3
State	Published - Jan 24 1992

ASJC Scopus subject areas

General Physics and Astronomy
Physical and Theoretical Chemistry

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@article{9d0a4ac9537c4015b078f3b27b79d5ab,

title = "Angular anisotropy of 2H NMR spectral densities in phospholipid bilayers containing cholesterol",

abstract = "Spin-lattice (R1Z) and quadrupolar order (R1Q) relaxation rates were measured for bilayers of macroscopically oriented 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC-d54) containing cholesterol (1:1 molar ratio), using inversion recovery and broadband Jeener-Broekaert pulse sequences, respectively. Anisotropic deuterium (2H) NMR spin relaxation data were obtained for the first time along the entire flexible acyl chains of the phospholipid molecules in the liquid-crystalline state. Individual spectral densities J1(ω0) and J2(2ω0) were calculated from these relaxation rates, and a strong dependence on the angle θ between the macroscopic bilayer normal and the static magnetic field was observed. The spectral densities exhibited opposite angular anisotropies, which were explained in terms of a simple rotational diffusion model for the molecular dynamics of membrane lipid constituents.",

author = "Trouard, \{Theodore P.\} and Alam, \{Todd M.\} and Jaroslav Zajicek and Brown, \{Michael F.\}",

note = "Funding Information: sequence,a nd Christian Mayer and Gerd Kothe for valuable discussions regardingt he preparation of oriented samples. This researchw as supported by NIH grants GM41413, EY03754, and EY06346 (TMA).",

year = "1992",

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doi = "10.1016/0009-2614(92)85154-3",

language = "English (US)",

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pages = "67--75",

journal = "Chemical Physics Letters",

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TY - JOUR

T1 - Angular anisotropy of 2H NMR spectral densities in phospholipid bilayers containing cholesterol

AU - Trouard, Theodore P.

AU - Alam, Todd M.

AU - Zajicek, Jaroslav

AU - Brown, Michael F.

N1 - Funding Information: sequence,a nd Christian Mayer and Gerd Kothe for valuable discussions regardingt he preparation of oriented samples. This researchw as supported by NIH grants GM41413, EY03754, and EY06346 (TMA).

PY - 1992/1/24

Y1 - 1992/1/24

N2 - Spin-lattice (R1Z) and quadrupolar order (R1Q) relaxation rates were measured for bilayers of macroscopically oriented 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC-d54) containing cholesterol (1:1 molar ratio), using inversion recovery and broadband Jeener-Broekaert pulse sequences, respectively. Anisotropic deuterium (2H) NMR spin relaxation data were obtained for the first time along the entire flexible acyl chains of the phospholipid molecules in the liquid-crystalline state. Individual spectral densities J1(ω0) and J2(2ω0) were calculated from these relaxation rates, and a strong dependence on the angle θ between the macroscopic bilayer normal and the static magnetic field was observed. The spectral densities exhibited opposite angular anisotropies, which were explained in terms of a simple rotational diffusion model for the molecular dynamics of membrane lipid constituents.

AB - Spin-lattice (R1Z) and quadrupolar order (R1Q) relaxation rates were measured for bilayers of macroscopically oriented 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC-d54) containing cholesterol (1:1 molar ratio), using inversion recovery and broadband Jeener-Broekaert pulse sequences, respectively. Anisotropic deuterium (2H) NMR spin relaxation data were obtained for the first time along the entire flexible acyl chains of the phospholipid molecules in the liquid-crystalline state. Individual spectral densities J1(ω0) and J2(2ω0) were calculated from these relaxation rates, and a strong dependence on the angle θ between the macroscopic bilayer normal and the static magnetic field was observed. The spectral densities exhibited opposite angular anisotropies, which were explained in terms of a simple rotational diffusion model for the molecular dynamics of membrane lipid constituents.

UR - https://www.scopus.com/pages/publications/0002179117

UR - https://www.scopus.com/pages/publications/0002179117#tab=citedBy

U2 - 10.1016/0009-2614(92)85154-3

DO - 10.1016/0009-2614(92)85154-3

M3 - Article

AN - SCOPUS:0002179117

SN - 0009-2614

VL - 189

SP - 67

EP - 75

JO - Chemical Physics Letters

JF - Chemical Physics Letters

IS - 1

ER -

Angular anisotropy of ²H NMR spectral densities in phospholipid bilayers containing cholesterol

Abstract

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