TY - JOUR
T1 - Angular anisotropy of 2H NMR spectral densities in phospholipid bilayers containing cholesterol
AU - Trouard, Theodore P.
AU - Alam, Todd M.
AU - Zajicek, Jaroslav
AU - Brown, Michael F.
N1 - Funding Information:
sequence,a nd Christian Mayer and Gerd Kothe for valuable discussions regardingt he preparation of oriented samples. This researchw as supported by NIH grants GM41413, EY03754, and EY06346 (TMA).
PY - 1992/1/24
Y1 - 1992/1/24
N2 - Spin-lattice (R1Z) and quadrupolar order (R1Q) relaxation rates were measured for bilayers of macroscopically oriented 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC-d54) containing cholesterol (1:1 molar ratio), using inversion recovery and broadband Jeener-Broekaert pulse sequences, respectively. Anisotropic deuterium (2H) NMR spin relaxation data were obtained for the first time along the entire flexible acyl chains of the phospholipid molecules in the liquid-crystalline state. Individual spectral densities J1(ω0) and J2(2ω0) were calculated from these relaxation rates, and a strong dependence on the angle θ between the macroscopic bilayer normal and the static magnetic field was observed. The spectral densities exhibited opposite angular anisotropies, which were explained in terms of a simple rotational diffusion model for the molecular dynamics of membrane lipid constituents.
AB - Spin-lattice (R1Z) and quadrupolar order (R1Q) relaxation rates were measured for bilayers of macroscopically oriented 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC-d54) containing cholesterol (1:1 molar ratio), using inversion recovery and broadband Jeener-Broekaert pulse sequences, respectively. Anisotropic deuterium (2H) NMR spin relaxation data were obtained for the first time along the entire flexible acyl chains of the phospholipid molecules in the liquid-crystalline state. Individual spectral densities J1(ω0) and J2(2ω0) were calculated from these relaxation rates, and a strong dependence on the angle θ between the macroscopic bilayer normal and the static magnetic field was observed. The spectral densities exhibited opposite angular anisotropies, which were explained in terms of a simple rotational diffusion model for the molecular dynamics of membrane lipid constituents.
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U2 - 10.1016/0009-2614(92)85154-3
DO - 10.1016/0009-2614(92)85154-3
M3 - Article
AN - SCOPUS:0002179117
SN - 0009-2614
VL - 189
SP - 67
EP - 75
JO - Chemical Physics Letters
JF - Chemical Physics Letters
IS - 1
ER -