TY - JOUR
T1 - Analyzing P-bodies and stress granules in saccharomyces cerevisiae
AU - Ross Buchan, J.
AU - Nissan, Tracy
AU - Parker, Roy
N1 - Funding Information:
We thank the members of the Parker lab for helpful discussions, particularly Carolyn Decker for proofreading and suggestions. NIH grant (R37 GM45443) and funds from the Howard Hughes Medical Institute supported this work. TN was supported in part by T32 CA09213.
PY - 2010
Y1 - 2010
N2 - Eukaryotic cells contain at least two types of cytoplasmic RNA-protein (RNP) granules that contain nontranslating mRNAs. One such RNP granule is a P-body, which contains translatlonally inactive mRNAs and proteins involved In mRNA degradation and translation repression. A second such RNP granule is a stress granule which also contains mRNAs, some RNA binding proteins and several translation initiation factors, suggesting these granules contain mRNAs stalled in translation initiation. In this chapter, we describe methods to analyze P-bodies and stress granules in Saccharomyces cerevisiae, including procedures to determine if a protein or mRNA can accumulate in either granule, if an environmental perturbation or mutation affects granule size and number, and granule quantification methods.
AB - Eukaryotic cells contain at least two types of cytoplasmic RNA-protein (RNP) granules that contain nontranslating mRNAs. One such RNP granule is a P-body, which contains translatlonally inactive mRNAs and proteins involved In mRNA degradation and translation repression. A second such RNP granule is a stress granule which also contains mRNAs, some RNA binding proteins and several translation initiation factors, suggesting these granules contain mRNAs stalled in translation initiation. In this chapter, we describe methods to analyze P-bodies and stress granules in Saccharomyces cerevisiae, including procedures to determine if a protein or mRNA can accumulate in either granule, if an environmental perturbation or mutation affects granule size and number, and granule quantification methods.
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U2 - 10.1016/S0076-6879(10)70025-2
DO - 10.1016/S0076-6879(10)70025-2
M3 - Article
C2 - 20946828
AN - SCOPUS:77956047670
SN - 0076-6879
VL - 470
SP - 619
EP - 640
JO - Methods in Enzymology
JF - Methods in Enzymology
IS - C
ER -