Analytical approaches to investigate transmitter content and release from single secretory vesicles

Donna M. Omiatek; Ann Sofie Cans; Michael L. Heien; Andrew G. Ewing

doi:10.1007/s00216-010-3698-4

Analytical approaches to investigate transmitter content and release from single secretory vesicles

Donna M. Omiatek, Ann Sofie Cans, Michael L. Heien, Andrew G. Ewing

Research output: Contribution to journal › Review article › peer-review

25 Scopus citations

Abstract

The vesicle serves as the primary intracellular unit for the highly efficient storage and release of chemical messengers triggered during signaling processes in the nervous system. This review highlights conventional and emerging analytical methods that have used microscopy, electrochemistry, and spectroscopy to resolve the location, time course, and quantal content characteristics of neurotransmitter release. Particular focus is on the investigation of the synaptic vesicle and its involvement in the fundamental molecular mechanisms of cell communication.

Original language	English (US)
Pages (from-to)	3269-3279
Number of pages	11
Journal	Analytical and bioanalytical chemistry
Volume	397
Issue number	8
DOIs	https://doi.org/10.1007/s00216-010-3698-4
State	Published - Aug 2010
Externally published	Yes

Keywords

Capillary electrophoresis
Carbon-fiber amperometry
Electrochemical cytometry
Exocytosis
Neurotransmitter
Vesicle

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry

Access to Document

10.1007/s00216-010-3698-4

Cite this

@article{f3cdd944ee124f1291acdd6d0e8c8746,

title = "Analytical approaches to investigate transmitter content and release from single secretory vesicles",

abstract = "The vesicle serves as the primary intracellular unit for the highly efficient storage and release of chemical messengers triggered during signaling processes in the nervous system. This review highlights conventional and emerging analytical methods that have used microscopy, electrochemistry, and spectroscopy to resolve the location, time course, and quantal content characteristics of neurotransmitter release. Particular focus is on the investigation of the synaptic vesicle and its involvement in the fundamental molecular mechanisms of cell communication.",

keywords = "Capillary electrophoresis, Carbon-fiber amperometry, Electrochemical cytometry, Exocytosis, Neurotransmitter, Vesicle",

author = "Omiatek, {Donna M.} and Cans, {Ann Sofie} and Heien, {Michael L.} and Ewing, {Andrew G.}",

note = "Funding Information: Acknowledgements The authors thank the National Institutes of Health (grant 5R01EB00352-10) and the Swedish National Science Foundation (VR) for support. A.G.E. is supported by a Marie Curie Chair from the European Union{\textquoteright}s Sixth Framework.",

year = "2010",

month = aug,

doi = "10.1007/s00216-010-3698-4",

language = "English (US)",

volume = "397",

pages = "3269--3279",

journal = "Analytical and bioanalytical chemistry",

issn = "1618-2642",

publisher = "Springer Verlag",

number = "8",

}

TY - JOUR

T1 - Analytical approaches to investigate transmitter content and release from single secretory vesicles

AU - Omiatek, Donna M.

AU - Cans, Ann Sofie

AU - Heien, Michael L.

AU - Ewing, Andrew G.

N1 - Funding Information: Acknowledgements The authors thank the National Institutes of Health (grant 5R01EB00352-10) and the Swedish National Science Foundation (VR) for support. A.G.E. is supported by a Marie Curie Chair from the European Union’s Sixth Framework.

PY - 2010/8

Y1 - 2010/8

N2 - The vesicle serves as the primary intracellular unit for the highly efficient storage and release of chemical messengers triggered during signaling processes in the nervous system. This review highlights conventional and emerging analytical methods that have used microscopy, electrochemistry, and spectroscopy to resolve the location, time course, and quantal content characteristics of neurotransmitter release. Particular focus is on the investigation of the synaptic vesicle and its involvement in the fundamental molecular mechanisms of cell communication.

AB - The vesicle serves as the primary intracellular unit for the highly efficient storage and release of chemical messengers triggered during signaling processes in the nervous system. This review highlights conventional and emerging analytical methods that have used microscopy, electrochemistry, and spectroscopy to resolve the location, time course, and quantal content characteristics of neurotransmitter release. Particular focus is on the investigation of the synaptic vesicle and its involvement in the fundamental molecular mechanisms of cell communication.

KW - Capillary electrophoresis

KW - Carbon-fiber amperometry

KW - Electrochemical cytometry

KW - Exocytosis

KW - Neurotransmitter

KW - Vesicle

UR - http://www.scopus.com/inward/record.url?scp=77956059189&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956059189&partnerID=8YFLogxK

U2 - 10.1007/s00216-010-3698-4

DO - 10.1007/s00216-010-3698-4

M3 - Review article

C2 - 20480152

AN - SCOPUS:77956059189

SN - 1618-2642

VL - 397

SP - 3269

EP - 3279

JO - Analytical and bioanalytical chemistry

JF - Analytical and bioanalytical chemistry

IS - 8

ER -

Analytical approaches to investigate transmitter content and release from single secretory vesicles

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this