TY - JOUR
T1 - Analysis of polyamines and acetyl derivatives by a single automated amino acid analyzer technique
AU - Russell, Diane Haddock
AU - Ellingson, Jeffrey D.
AU - Davis, Thomas P.
PY - 1983
Y1 - 1983
N2 - In a single, rapid and precise analysis, monoacetylputrescine, N8-acetylspermidine, N1acetylspermidine, putrescine, spermidine, and spermine can be separated using a five-buffer system on an automatic amino acid analyzer. This method allows, for the first time, the separation of all the known acetyl derivatives of putrescine and spermidine as well as the parent compounds in urine and tissues with a single automated procedure. The method has been applied to the analysis of biological samples from normal volunteers, cancer patients and a rat liver supernatant. Mass spectral confirmation was obtained for each compound.
AB - In a single, rapid and precise analysis, monoacetylputrescine, N8-acetylspermidine, N1acetylspermidine, putrescine, spermidine, and spermine can be separated using a five-buffer system on an automatic amino acid analyzer. This method allows, for the first time, the separation of all the known acetyl derivatives of putrescine and spermidine as well as the parent compounds in urine and tissues with a single automated procedure. The method has been applied to the analysis of biological samples from normal volunteers, cancer patients and a rat liver supernatant. Mass spectral confirmation was obtained for each compound.
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U2 - 10.1016/S0378-4347(00)80948-2
DO - 10.1016/S0378-4347(00)80948-2
M3 - Article
C2 - 6863443
AN - SCOPUS:0020539024
SN - 0378-4347
VL - 273
SP - 263
EP - 274
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - 2
ER -