Analysis of p-chlorobenzoic acid in water by liquid chromatography-tandem mass spectrometry

Brett J. Vanderford, Fernando L. Rosario-Ortiz, Shane A. Snyder

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

para-Chlorobenzoic acid (p-CBA) is typically used as a probe compound to indirectly quantify hydroxyl radicals formed during advanced oxidation processes used in drinking water and wastewater treatment. A method has been developed for the sensitive analysis of p-CBA in water using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A reporting limit in water of 100 ng/L was determined for the method, which is 40-fold lower than the 4.0 μg/L reporting limit of the widely used liquid chromatography with UV detection (LC-UV) method. The method was found to be robust in difficult matrices such as wastewater and highly selective, unlike LC-UV which relies on non-specific detection at 234 nm. The detection of p-CBA below 1 μg/L during bench-scale ozonation of wastewater after hydrogen peroxide addition was demonstrated. Duplicate samples were analyzed by LC-MS/MS and LC-UV and results were found to be comparable at concentrations quantifiable by both methods.

Original languageEnglish (US)
Pages (from-to)219-223
Number of pages5
JournalJournal of Chromatography A
Volume1164
Issue number1-2
DOIs
StatePublished - Sep 14 2007

Keywords

  • LC-MS/MS
  • Liquid chromatography
  • Mass spectrometry
  • p-Chlorobenzoic acid (p-CBA)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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