Analysis of MRP mRNA in mitoxantrone-selected, multidrug-resistant human tumor cells

Bernard W. Futscher; Mohammad R. Abbaszadegan; Frederick Domann; William S. Dalton

doi:10.1016/0006-2952(94)90538-X

Analysis of MRP mRNA in mitoxantrone-selected, multidrug-resistant human tumor cells

Bernard W. Futscher
, Mohammad R. Abbaszadegan
, Frederick Domann
, William S. Dalton

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

MRP, a gene recently isolated from a non-P-glycoprotein-mediated multidrug-resistant small cell lung cancer line, is a candidate multidrug-resistance gene. Mitoxantrone, an anthracenedione antitumor agent, frequently selects for non-P-glycoprotein-mediated multidrug resistance in in vitro models. To determine whether mitoxantrone-selected multidrug resistance wasdue to overexpression of MRP, we examined the expression of MRP in four mitoxantrone-selected, multidrug-resistant human tumor cell lines, using a reverse transcriptase/polymerase chain reaction assay. Results from these experiments suggest that overexpression of MRP does not appear to play a primary role in mitoxantrone-selected multidrug resistance in these cell lines, and that other novel drug-resistance mechanisms are likely.

Original language	English (US)
Pages (from-to)	1601-1606
Number of pages	6
Journal	Biochemical Pharmacology
Volume	47
Issue number	9
DOIs	https://doi.org/10.1016/0006-2952(94)90538-X
State	Published - Apr 29 1994

Keywords

MRP
PCR
drug resistance
human tumor cell lines
mRNA
mitoxantrone

ASJC Scopus subject areas

Biochemistry
Pharmacology

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10.1016/0006-2952(94)90538-X

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@article{546710b2a5a542ac9eeeab73884f5432,

title = "Analysis of MRP mRNA in mitoxantrone-selected, multidrug-resistant human tumor cells",

abstract = "MRP, a gene recently isolated from a non-P-glycoprotein-mediated multidrug-resistant small cell lung cancer line, is a candidate multidrug-resistance gene. Mitoxantrone, an anthracenedione antitumor agent, frequently selects for non-P-glycoprotein-mediated multidrug resistance in in vitro models. To determine whether mitoxantrone-selected multidrug resistance wasdue to overexpression of MRP, we examined the expression of MRP in four mitoxantrone-selected, multidrug-resistant human tumor cell lines, using a reverse transcriptase/polymerase chain reaction assay. Results from these experiments suggest that overexpression of MRP does not appear to play a primary role in mitoxantrone-selected multidrug resistance in these cell lines, and that other novel drug-resistance mechanisms are likely.",

keywords = "MRP, PCR, drug resistance, human tumor cell lines, mRNA, mitoxantrone",

author = "Futscher, \{Bernard W.\} and Abbaszadegan, \{Mohammad R.\} and Frederick Domann and Dalton, \{William S.\}",

note = "Funding Information: Acknowledgernents--The authors would like to thank Jeffery S. Painter for northern blot analysis of MRP, Mary Gleason-Guzman for tissue culture expertise, and Nils Foley for western blot analysis of PGP. This work was supported in part by NC1 Grants 17094 and 43043 to W.S.D.",

year = "1994",

month = apr,

day = "29",

doi = "10.1016/0006-2952(94)90538-X",

language = "English (US)",

volume = "47",

pages = "1601--1606",

journal = "Biochemical Pharmacology",

issn = "0006-2952",

publisher = "Elsevier Inc.",

number = "9",

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TY - JOUR

T1 - Analysis of MRP mRNA in mitoxantrone-selected, multidrug-resistant human tumor cells

AU - Futscher, Bernard W.

AU - Abbaszadegan, Mohammad R.

AU - Domann, Frederick

AU - Dalton, William S.

N1 - Funding Information: Acknowledgernents--The authors would like to thank Jeffery S. Painter for northern blot analysis of MRP, Mary Gleason-Guzman for tissue culture expertise, and Nils Foley for western blot analysis of PGP. This work was supported in part by NC1 Grants 17094 and 43043 to W.S.D.

PY - 1994/4/29

Y1 - 1994/4/29

N2 - MRP, a gene recently isolated from a non-P-glycoprotein-mediated multidrug-resistant small cell lung cancer line, is a candidate multidrug-resistance gene. Mitoxantrone, an anthracenedione antitumor agent, frequently selects for non-P-glycoprotein-mediated multidrug resistance in in vitro models. To determine whether mitoxantrone-selected multidrug resistance wasdue to overexpression of MRP, we examined the expression of MRP in four mitoxantrone-selected, multidrug-resistant human tumor cell lines, using a reverse transcriptase/polymerase chain reaction assay. Results from these experiments suggest that overexpression of MRP does not appear to play a primary role in mitoxantrone-selected multidrug resistance in these cell lines, and that other novel drug-resistance mechanisms are likely.

AB - MRP, a gene recently isolated from a non-P-glycoprotein-mediated multidrug-resistant small cell lung cancer line, is a candidate multidrug-resistance gene. Mitoxantrone, an anthracenedione antitumor agent, frequently selects for non-P-glycoprotein-mediated multidrug resistance in in vitro models. To determine whether mitoxantrone-selected multidrug resistance wasdue to overexpression of MRP, we examined the expression of MRP in four mitoxantrone-selected, multidrug-resistant human tumor cell lines, using a reverse transcriptase/polymerase chain reaction assay. Results from these experiments suggest that overexpression of MRP does not appear to play a primary role in mitoxantrone-selected multidrug resistance in these cell lines, and that other novel drug-resistance mechanisms are likely.

KW - MRP

KW - PCR

KW - drug resistance

KW - human tumor cell lines

KW - mRNA

KW - mitoxantrone

UR - https://www.scopus.com/pages/publications/0028239105

UR - https://www.scopus.com/pages/publications/0028239105#tab=citedBy

U2 - 10.1016/0006-2952(94)90538-X

DO - 10.1016/0006-2952(94)90538-X

M3 - Article

C2 - 8185674

AN - SCOPUS:0028239105

SN - 0006-2952

VL - 47

SP - 1601

EP - 1606

JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

IS - 9

ER -

Analysis of MRP mRNA in mitoxantrone-selected, multidrug-resistant human tumor cells

Abstract

Keywords

ASJC Scopus subject areas

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