@inbook{04ce1c94d5684b56884ce56bb4c04987,
title = "Analysis of Membrane-Bound Organelles",
abstract = "This chapter describes methods for studying membrane traffic and organelle biogenesis in Caenorhabditis elegans. These processes have traditionally been studied with yeast or mammalian cells, but C. elegans is emerging as an attractive alternative model system for cell biologists. C. elegans is well known for the ease of manipulation through classic and molecular genetic techniques. In addition, C. elegans is transparent, so fluorescent proteins can be observed in live animals. These properties have aided the development of functional assays for tracking cell biological processes in situ. Localization results obtained with fluorescent proteins can be validated with immunofluorescence and with biochemical methods, such as subcellular fractionation, adapted from methods developed for other organisms. C. elegans thus combines powerful genetics with a range of cell biological techniques to study subcellular processes in a tractable multicellular organism.",
keywords = "Construct, Endocytosis, Epitope, Lysosomal, Mapping, Photobleaching",
author = "Hanna Fares and {van der Bliek}, {Alexander M.}",
note = "Funding Information: We would like to thank the members of our labs for their many contributions to the development of these protocols. Work in the Fares lab is supported by an Arizona Biomedical Research Commission (grant 8-006) and the van der Bliek lab is supported by a grant from the National Institutes of Health (GM051866).",
year = "2012",
doi = "10.1016/B978-0-12-394620-1.00008-4",
language = "English (US)",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc.",
pages = "239--263",
booktitle = "Methods in Cell Biology",
}