TY - JOUR
T1 - Analysis of Histone H5 and Globin mRNAs in Chick‐Embryo Development
AU - COLMAN, Alan
AU - KRIEG, Paul
AU - WELLS, Julian R.E.
PY - 1983/4
Y1 - 1983/4
N2 - Transcripts from histone H5 and α and β chicken globin genes were analysed in total RNA extracted from 2–6‐day‐old chick embryos. Denatured RNA, separated on gels, was transferred to either aminophenylthioether (APT) paper or to nitrocellulose and was detected with 32P‐labelled recombinant DNA probes. Good resolution was achieved with methyl mercury hydroxide gels and aminophenylthioether transfer, allowing estimation of H5 and α and β globin mRNA sizes. With glyoxal gels and nitrocellulose transfer, α and β globin mRNAs were not resolved, but an increased sensitivity of detection of at least 10‐fold was achieved. Levels of H5 and α globin mRNAs increased in parallel over 2–6 days. The appearance of definitive red cells in 6‐day embryos was indicated by detection of adult β globin transcripts at this stage.
AB - Transcripts from histone H5 and α and β chicken globin genes were analysed in total RNA extracted from 2–6‐day‐old chick embryos. Denatured RNA, separated on gels, was transferred to either aminophenylthioether (APT) paper or to nitrocellulose and was detected with 32P‐labelled recombinant DNA probes. Good resolution was achieved with methyl mercury hydroxide gels and aminophenylthioether transfer, allowing estimation of H5 and α and β globin mRNA sizes. With glyoxal gels and nitrocellulose transfer, α and β globin mRNAs were not resolved, but an increased sensitivity of detection of at least 10‐fold was achieved. Levels of H5 and α globin mRNAs increased in parallel over 2–6 days. The appearance of definitive red cells in 6‐day embryos was indicated by detection of adult β globin transcripts at this stage.
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U2 - 10.1111/j.1432-1033.1983.tb07297.x
DO - 10.1111/j.1432-1033.1983.tb07297.x
M3 - Article
C2 - 6840066
AN - SCOPUS:0021095298
SN - 0014-2956
VL - 131
SP - 551
EP - 554
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 3
ER -