TY - JOUR
T1 - Analysis of gene function using DNA microarrays
AU - Capaldi, Andrew P.
PY - 2010
Y1 - 2010
N2 - This chapter provides a guide to analyzing gene function using DNA microarrays. First, I discuss the design and interpretation of experiments where gene expression levels in mutant and wild-type strains are compared. I then provide a detailed description of the protocols for isolating mRNA from yeast cells, converting the RNA into dye-labeled cDNA, and hybridizing these samples to a microarray. Finally, I discuss methods for washing, scanning, and analyzing the arrays. Emphasis is placed on describing approaches and techniques that help to minimize the artifacts and noise that so often plague microarray data.
AB - This chapter provides a guide to analyzing gene function using DNA microarrays. First, I discuss the design and interpretation of experiments where gene expression levels in mutant and wild-type strains are compared. I then provide a detailed description of the protocols for isolating mRNA from yeast cells, converting the RNA into dye-labeled cDNA, and hybridizing these samples to a microarray. Finally, I discuss methods for washing, scanning, and analyzing the arrays. Emphasis is placed on describing approaches and techniques that help to minimize the artifacts and noise that so often plague microarray data.
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U2 - 10.1016/S0076-6879(10)70001-X
DO - 10.1016/S0076-6879(10)70001-X
M3 - Article
C2 - 20946804
AN - SCOPUS:77956055139
SN - 0076-6879
VL - 470
SP - 3
EP - 17
JO - Methods in Enzymology
JF - Methods in Enzymology
IS - C
ER -