TY - GEN
T1 - An SU-8 based fluidic immuno-spectroscopic lab-on-a-chip for rapid quantitative detection of biomolecules
AU - Jiang, Linan
AU - Gerhardt, Karl P.
AU - Myer, Brian
AU - Zohar, Yitshak
AU - Pau, Stanley
PY - 2008
Y1 - 2008
N2 - A 30mm-long multimode waveguide, 40μm wide and 40μm high, is fabricated on a silicon wafer using polymer SU-8 as the core and liquid buffer as the cladding. Antibodies are successfully immobilized on the SU-8 surface designated for binding target antigens dispersed in the buffer solution. Evanescent-wave spectroscopy is performed by exciting the fluorescently-labeled antigens, bound to the waveguide surface within its evanescence field, and measuring emission light intensity. This evanescent-wave biosensor detects specific molecular interaction; its output depends on antigen concentration with a sensitivity of 40 light counts per 1μg/ml.
AB - A 30mm-long multimode waveguide, 40μm wide and 40μm high, is fabricated on a silicon wafer using polymer SU-8 as the core and liquid buffer as the cladding. Antibodies are successfully immobilized on the SU-8 surface designated for binding target antigens dispersed in the buffer solution. Evanescent-wave spectroscopy is performed by exciting the fluorescently-labeled antigens, bound to the waveguide surface within its evanescence field, and measuring emission light intensity. This evanescent-wave biosensor detects specific molecular interaction; its output depends on antigen concentration with a sensitivity of 40 light counts per 1μg/ml.
UR - http://www.scopus.com/inward/record.url?scp=50149084100&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=50149084100&partnerID=8YFLogxK
U2 - 10.1109/MEMSYS.2008.4443628
DO - 10.1109/MEMSYS.2008.4443628
M3 - Conference contribution
AN - SCOPUS:50149084100
SN - 9781424417933
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 204
EP - 207
BT - MEMS 2008 Tucson - 21st IEEE International Conference on Micro Electro Mechanical Systems
T2 - 21st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2008 Tucson
Y2 - 13 January 2008 through 17 January 2008
ER -