TY - JOUR
T1 - An NLR paralog Pit2 generated from tandem duplication of Pit1 fine-tunes Pit1 localization and function
AU - Li, Yuying
AU - Wang, Qiong
AU - Jia, Huimin
AU - Ishikawa, Kazuya
AU - Kosami, Ken Ichi
AU - Ueba, Takahiro
AU - Tsujimoto, Atsumi
AU - Yamanaka, Miki
AU - Yabumoto, Yasuyuki
AU - Miki, Daisuke
AU - Sasaki, Eriko
AU - Fukao, Yoichiro
AU - Fujiwara, Masayuki
AU - Kaneko-Kawano, Takako
AU - Tan, Li
AU - Kojima, Chojiro
AU - Wing, Rod A.
AU - Sebastian, Alfino
AU - Nishimura, Hideki
AU - Fukada, Fumi
AU - Niu, Qingfeng
AU - Shimizu, Motoki
AU - Yoshida, Kentaro
AU - Terauchi, Ryohei
AU - Shimamoto, Ko
AU - Kawano, Yoji
N1 - Publisher Copyright:
© The Author(s) 2024.
PY - 2024/12
Y1 - 2024/12
N2 - NLR family proteins act as intracellular receptors. Gene duplication amplifies the number of NLR genes, and subsequent mutations occasionally provide modifications to the second gene that benefits immunity. However, evolutionary processes after gene duplication and functional relationships between duplicated NLRs remain largely unclear. Here, we report that the rice NLR protein Pit1 is associated with its paralogue Pit2. The two are required for the resistance to rice blast fungus but have different functions: Pit1 induces cell death, while Pit2 competitively suppresses Pit1-mediated cell death. During evolution, the suppression of Pit1 by Pit2 was probably generated through positive selection on two fate-determining residues in the NB-ARC domain of Pit2, which account for functional differences between Pit1 and Pit2. Consequently, Pit2 lost its plasma membrane localization but acquired a new function to interfere with Pit1 in the cytosol. These findings illuminate the evolutionary trajectory of tandemly duplicated NLR genes after gene duplication.
AB - NLR family proteins act as intracellular receptors. Gene duplication amplifies the number of NLR genes, and subsequent mutations occasionally provide modifications to the second gene that benefits immunity. However, evolutionary processes after gene duplication and functional relationships between duplicated NLRs remain largely unclear. Here, we report that the rice NLR protein Pit1 is associated with its paralogue Pit2. The two are required for the resistance to rice blast fungus but have different functions: Pit1 induces cell death, while Pit2 competitively suppresses Pit1-mediated cell death. During evolution, the suppression of Pit1 by Pit2 was probably generated through positive selection on two fate-determining residues in the NB-ARC domain of Pit2, which account for functional differences between Pit1 and Pit2. Consequently, Pit2 lost its plasma membrane localization but acquired a new function to interfere with Pit1 in the cytosol. These findings illuminate the evolutionary trajectory of tandemly duplicated NLR genes after gene duplication.
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U2 - 10.1038/s41467-024-48943-5
DO - 10.1038/s41467-024-48943-5
M3 - Article
C2 - 38816417
AN - SCOPUS:85194992176
SN - 2041-1723
VL - 15
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 4610
ER -