An enhancer responsible for activating transcription at the midblastula transition in Xenopus development

P. A. Krieg; D. A. Melton

doi:10.1073/pnas.84.8.2331

An enhancer responsible for activating transcription at the midblastula transition in Xenopus development

P. A. Krieg, D. A. Melton

Cellular and Molecular Medicine

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Abstract

Transcripts from the Xenopus gene GS17 are absent from oocytes and first appear at the midblastula transition (MBT), about 8 hr after fertilization. Injection experiments with deletions of the GS17 gene and GS17-β-globin hybrid genes show that a relatively short sequence of 74 base pairs is sufficient to activate, in cis, transcription at the MBT. This 74-base sequence is located about 700 bases upstream of the GS17 promoter and has the properties of an enhancer element.

Original language	English (US)
Pages (from-to)	2331-2335
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	84
Issue number	8
DOIs	https://doi.org/10.1073/pnas.84.8.2331
State	Published - 1987

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abstract = "Transcripts from the Xenopus gene GS17 are absent from oocytes and first appear at the midblastula transition (MBT), about 8 hr after fertilization. Injection experiments with deletions of the GS17 gene and GS17-β-globin hybrid genes show that a relatively short sequence of 74 base pairs is sufficient to activate, in cis, transcription at the MBT. This 74-base sequence is located about 700 bases upstream of the GS17 promoter and has the properties of an enhancer element.",

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AU - Krieg, P. A.

AU - Melton, D. A.

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AB - Transcripts from the Xenopus gene GS17 are absent from oocytes and first appear at the midblastula transition (MBT), about 8 hr after fertilization. Injection experiments with deletions of the GS17 gene and GS17-β-globin hybrid genes show that a relatively short sequence of 74 base pairs is sufficient to activate, in cis, transcription at the MBT. This 74-base sequence is located about 700 bases upstream of the GS17 promoter and has the properties of an enhancer element.

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ER -

An enhancer responsible for activating transcription at the midblastula transition in Xenopus development

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