An Electron Microscope‐Cytochemical Method for Differentiating Membranes of Host Red Cells and Malaria Parasites

THOMAS M. SEED; MASAMICHI AIKAWA; CHARLES R. STERLING

doi:10.1111/j.1550-7408.1973.tb03583.x

An Electron Microscope‐Cytochemical Method for Differentiating Membranes of Host Red Cells and Malaria Parasites

THOMAS M. SEED, MASAMICHI AIKAWA, CHARLES R. STERLING

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Limiting membranes of malaria parasites and host red cells stain differently when exposed to positively charged iron colloid. Negatively charged red cell membranes avidly bind colloid, whereas parasite membranes do not. This selectivity in colloidal iron uptake by the 2 types of membranes can be utilized as an aid in discerning the amounts of contaminating host cell membranes in “free” malaria parasite preparations and in related cell‐free membrane extracts.

Original language	English (US)
Pages (from-to)	603-605
Number of pages	3
Journal	The Journal of protozoology
Volume	20
Issue number	5
DOIs	https://doi.org/10.1111/j.1550-7408.1973.tb03583.x
State	Published - Nov 1973
Externally published	Yes

Keywords

Plasmodium berghei
colloidal iron
cytochemistry
electron microscopy
host erythrocytes
malaria parasite

ASJC Scopus subject areas

Parasitology

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10.1111/j.1550-7408.1973.tb03583.x

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title = "An Electron Microscope‐Cytochemical Method for Differentiating Membranes of Host Red Cells and Malaria Parasites",

abstract = "Limiting membranes of malaria parasites and host red cells stain differently when exposed to positively charged iron colloid. Negatively charged red cell membranes avidly bind colloid, whereas parasite membranes do not. This selectivity in colloidal iron uptake by the 2 types of membranes can be utilized as an aid in discerning the amounts of contaminating host cell membranes in “free” malaria parasite preparations and in related cell‐free membrane extracts.",

keywords = "Plasmodium berghei, colloidal iron, cytochemistry, electron microscopy, host erythrocytes, malaria parasite",

author = "SEED, {THOMAS M.} and MASAMICHI AIKAWA and STERLING, {CHARLES R.}",

year = "1973",

month = nov,

doi = "10.1111/j.1550-7408.1973.tb03583.x",

language = "English (US)",

volume = "20",

pages = "603--605",

journal = "The Journal of protozoology",

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T1 - An Electron Microscope‐Cytochemical Method for Differentiating Membranes of Host Red Cells and Malaria Parasites

AU - SEED, THOMAS M.

AU - AIKAWA, MASAMICHI

AU - STERLING, CHARLES R.

PY - 1973/11

Y1 - 1973/11

N2 - Limiting membranes of malaria parasites and host red cells stain differently when exposed to positively charged iron colloid. Negatively charged red cell membranes avidly bind colloid, whereas parasite membranes do not. This selectivity in colloidal iron uptake by the 2 types of membranes can be utilized as an aid in discerning the amounts of contaminating host cell membranes in “free” malaria parasite preparations and in related cell‐free membrane extracts.

AB - Limiting membranes of malaria parasites and host red cells stain differently when exposed to positively charged iron colloid. Negatively charged red cell membranes avidly bind colloid, whereas parasite membranes do not. This selectivity in colloidal iron uptake by the 2 types of membranes can be utilized as an aid in discerning the amounts of contaminating host cell membranes in “free” malaria parasite preparations and in related cell‐free membrane extracts.

KW - Plasmodium berghei

KW - colloidal iron

KW - cytochemistry

KW - electron microscopy

KW - host erythrocytes

KW - malaria parasite

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VL - 20

SP - 603

EP - 605

JO - The Journal of protozoology

JF - The Journal of protozoology

IS - 5

ER -

An Electron Microscope‐Cytochemical Method for Differentiating Membranes of Host Red Cells and Malaria Parasites

Abstract

Keywords

ASJC Scopus subject areas

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