An ecdysterone-responsive puff site in Drosophila contains a cluster of seven differentially regulated genes

Linda L. Restifo; Gregory M. Guild

doi:10.1016/S0022-2836(86)80002-X

An ecdysterone-responsive puff site in Drosophila contains a cluster of seven differentially regulated genes

Linda L. Restifo, Gregory M. Guild

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

We have determined the molecular organization of an ecdysterone-responsive puff site in Drosophila melanogaster. The 71E puff site contains a tightly linked cluster of at least seven genes within a neighborhood of 10 × 10³ base-pairs. All the genes are expressed in a tissue-specific manner in either the larval or the prepupal salivary gland. However, these genes can be divided into two groups on the basis of their temporal pattern of transcription. Six of the genes are expressed only in prepupal salivary glands and are arranged as three divergently transcribed pairs. Nestled within this region is one gene expressed primarily in late third-instar salivary glands. We conclude that this developmentally complex puff site contains six members of the ecdysterone-induced "late"-gene set and one member of the ecdysterone-regulated "intermolt"-gene set. Additional complexity is found at the transcript level: a heterogeneously sized population of RNA molecules arises from each of the seven genes.

Original language	English (US)
Pages (from-to)	517-528
Number of pages	12
Journal	Journal of Molecular Biology
Volume	188
Issue number	4
DOIs	https://doi.org/10.1016/S0022-2836(86)80002-X
State	Published - Apr 20 1986
Externally published	Yes

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1016/S0022-2836(86)80002-X

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title = "An ecdysterone-responsive puff site in Drosophila contains a cluster of seven differentially regulated genes",

abstract = "We have determined the molecular organization of an ecdysterone-responsive puff site in Drosophila melanogaster. The 71E puff site contains a tightly linked cluster of at least seven genes within a neighborhood of 10 × 103 base-pairs. All the genes are expressed in a tissue-specific manner in either the larval or the prepupal salivary gland. However, these genes can be divided into two groups on the basis of their temporal pattern of transcription. Six of the genes are expressed only in prepupal salivary glands and are arranged as three divergently transcribed pairs. Nestled within this region is one gene expressed primarily in late third-instar salivary glands. We conclude that this developmentally complex puff site contains six members of the ecdysterone-induced {"}late{"}-gene set and one member of the ecdysterone-regulated {"}intermolt{"}-gene set. Additional complexity is found at the transcript level: a heterogeneously sized population of RNA molecules arises from each of the seven genes.",

author = "Restifo, {Linda L.} and Guild, {Gregory M.}",

note = "Funding Information: We thank Lynn Zukosky for her excellent technical assistance and expert graphic arts skills in the construction of the Figures, M. Wolfner for the generous gift of the eDNA clones and for discussions during the early phase of this work, R. Pollack for constructing many of the subclones, P. Dunn for fly care, M. Ashburner and K. Tartof for supplying fly stocks, D. Eickbush and P. Cherbas for discussion of unpublished results, T. Vogt for bringing the formaldehyde denaturation protocol to our attention, and A. Chao and E. Shore for discussions and helpful comments on the manuscript. This work was supported by a grant from the National Institutes of Health (5-R01 GM28228). L.L.R. was supported by a Medical Scientist Training Program grant (5-T32 GM07170).",

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AU - Restifo, Linda L.

AU - Guild, Gregory M.

N1 - Funding Information: We thank Lynn Zukosky for her excellent technical assistance and expert graphic arts skills in the construction of the Figures, M. Wolfner for the generous gift of the eDNA clones and for discussions during the early phase of this work, R. Pollack for constructing many of the subclones, P. Dunn for fly care, M. Ashburner and K. Tartof for supplying fly stocks, D. Eickbush and P. Cherbas for discussion of unpublished results, T. Vogt for bringing the formaldehyde denaturation protocol to our attention, and A. Chao and E. Shore for discussions and helpful comments on the manuscript. This work was supported by a grant from the National Institutes of Health (5-R01 GM28228). L.L.R. was supported by a Medical Scientist Training Program grant (5-T32 GM07170).

PY - 1986/4/20

Y1 - 1986/4/20

N2 - We have determined the molecular organization of an ecdysterone-responsive puff site in Drosophila melanogaster. The 71E puff site contains a tightly linked cluster of at least seven genes within a neighborhood of 10 × 103 base-pairs. All the genes are expressed in a tissue-specific manner in either the larval or the prepupal salivary gland. However, these genes can be divided into two groups on the basis of their temporal pattern of transcription. Six of the genes are expressed only in prepupal salivary glands and are arranged as three divergently transcribed pairs. Nestled within this region is one gene expressed primarily in late third-instar salivary glands. We conclude that this developmentally complex puff site contains six members of the ecdysterone-induced "late"-gene set and one member of the ecdysterone-regulated "intermolt"-gene set. Additional complexity is found at the transcript level: a heterogeneously sized population of RNA molecules arises from each of the seven genes.

AB - We have determined the molecular organization of an ecdysterone-responsive puff site in Drosophila melanogaster. The 71E puff site contains a tightly linked cluster of at least seven genes within a neighborhood of 10 × 103 base-pairs. All the genes are expressed in a tissue-specific manner in either the larval or the prepupal salivary gland. However, these genes can be divided into two groups on the basis of their temporal pattern of transcription. Six of the genes are expressed only in prepupal salivary glands and are arranged as three divergently transcribed pairs. Nestled within this region is one gene expressed primarily in late third-instar salivary glands. We conclude that this developmentally complex puff site contains six members of the ecdysterone-induced "late"-gene set and one member of the ecdysterone-regulated "intermolt"-gene set. Additional complexity is found at the transcript level: a heterogeneously sized population of RNA molecules arises from each of the seven genes.

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An ecdysterone-responsive puff site in Drosophila contains a cluster of seven differentially regulated genes

Abstract

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