An Assay Suitable for High Throughput Screening of Anti-Influenza Drugs

Lili Mao; Jun Wang; William F. DeGrado; Masayori Inouye

doi:10.1371/journal.pone.0054070

An Assay Suitable for High Throughput Screening of Anti-Influenza Drugs

Lili Mao, Jun Wang, William F. DeGrado, Masayori Inouye

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

We developed a novel drug screening system for anti-influenza A virus by targeting the M2 proton channel. In the SPP (Single Protein Production) system, E. coli cell growth occurs only in the presence of effective M2 channel inhibitors, and thus simple measurement of cell growth was used as readouts for drug screening. Two potential inhibitors for M2 (V27A) mutant were verified using this method, which inhibit both the mutant and wild-type M2 channels.

Original language	English (US)
Article number	e54070
Journal	PloS one
Volume	8
Issue number	1
DOIs	https://doi.org/10.1371/journal.pone.0054070
State	Published - Jan 17 2013
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences
General

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An Assay Suitable for High Throughput Screening of Anti-Influenza Drugs

Abstract

ASJC Scopus subject areas

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