Amplifying small amounts of tumor DNA allows detection of DNA copy number aberrations with array-CGH

Lars Prestegarden; Anjan Misra; Marcus L. Ware; Ru Fang Yeh; Rolf Bjerkvig; Burt G. Feuerstein

doi:10.2144/000112760

Amplifying small amounts of tumor DNA allows detection of DNA copy number aberrations with array-CGH

Lars Prestegarden, Anjan Misra, Marcus L. Ware, Ru Fang Yeh, Rolf Bjerkvig, Burt G. Feuerstein

Research output: Contribution to journal › Article › peer-review

Abstract

Array comparative genomic hybridization (aCGH) is a powerful tool to detect relative DNA copy number at a resolution limited only by the coverage of bacterial artificial chromosomes (BACs) used to print the genomic array. The amount of DNA needed to perform a reliable aCGH analysis has been a limiting factor, especially on minute tissue samples where limited DNA is available. Here we report a simple, highly sensitive and reliable aCGH method to analyze samples of no more than 1 ng genomic DNA. The speed and simplicity of the technique are ideal for studies on small clinical samples such as needle biopsies.

Original language	English (US)
Pages (from-to)	3-6
Number of pages	4
Journal	BioTechniques
Volume	44
Issue number	7
DOIs	https://doi.org/10.2144/000112760
State	Published - Jun 2008
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
General Biochemistry, Genetics and Molecular Biology

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abstract = "Array comparative genomic hybridization (aCGH) is a powerful tool to detect relative DNA copy number at a resolution limited only by the coverage of bacterial artificial chromosomes (BACs) used to print the genomic array. The amount of DNA needed to perform a reliable aCGH analysis has been a limiting factor, especially on minute tissue samples where limited DNA is available. Here we report a simple, highly sensitive and reliable aCGH method to analyze samples of no more than 1 ng genomic DNA. The speed and simplicity of the technique are ideal for studies on small clinical samples such as needle biopsies.",

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AU - Bjerkvig, Rolf

AU - Feuerstein, Burt G.

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AB - Array comparative genomic hybridization (aCGH) is a powerful tool to detect relative DNA copy number at a resolution limited only by the coverage of bacterial artificial chromosomes (BACs) used to print the genomic array. The amount of DNA needed to perform a reliable aCGH analysis has been a limiting factor, especially on minute tissue samples where limited DNA is available. Here we report a simple, highly sensitive and reliable aCGH method to analyze samples of no more than 1 ng genomic DNA. The speed and simplicity of the technique are ideal for studies on small clinical samples such as needle biopsies.

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Amplifying small amounts of tumor DNA allows detection of DNA copy number aberrations with array-CGH

Abstract

ASJC Scopus subject areas

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