TY - JOUR
T1 - Alzheimer disease macrophages shuttle amyloid-beta from neurons to vessels, contributing to amyloid angiopathy
AU - Zaghi, Justin
AU - Goldenson, Ben
AU - Inayathullah, Mohammed
AU - Lossinsky, Albert S.
AU - Masoumi, Ava
AU - Avagyan, Hripsime
AU - Mahanian, Michelle
AU - Bernas, Michael J
AU - Weinand, Martin
AU - Rosenthal, Mark J.
AU - Espinosa-Jeffrey, Araceli
AU - Vellis, Jean
AU - Teplow, David B.
AU - Fiala, Milan
N1 - Funding Information:
ConXict of interest statement This work was supported by the
PY - 2009
Y1 - 2009
N2 - Neuronal accumulation of oligomeric amyloid-β (αβ) is considered the proximal cause of neuronal demise in Alzheimer disease (AD) patients. Blood-borne macrophages might reduce Aβ stress to neurons by immigration into the brain and phagocytosis of αβ. We tested migration and export across a blood-brain barrier model, and phagocytosis and clearance of αβ by AD and normal subjects' macrophages. Both AD and normal macrophages were inhibited in αβ export across the blood-brain barrier due to adherence of Aβ-engorged macrophages to the endothelial layer. In comparison to normal subjects' macrophages, AD macrophages ingested and cleared less αβ, and underwent apoptosis upon exposure to soluble, protofibrillar, or fibrillar αβ. Confocal microscopy of stained AD brain sections revealed oligomeric Aβ in neurons and apoptotic macrophages, which surrounded and infiltrated congophilic microvessels, and fibrillar Aβ in plaques and microvessel walls. After incubation with AD brain sections, normal subjects' monocytes intruded into neurons and uploaded oligomeric Aβ. In conclusion, in patients with AD, macrophages appear to shuttle Aβ from neurons to vessels where their apoptosis may release fibrillar Aβ, contributing to cerebral amyloid angiopathy.
AB - Neuronal accumulation of oligomeric amyloid-β (αβ) is considered the proximal cause of neuronal demise in Alzheimer disease (AD) patients. Blood-borne macrophages might reduce Aβ stress to neurons by immigration into the brain and phagocytosis of αβ. We tested migration and export across a blood-brain barrier model, and phagocytosis and clearance of αβ by AD and normal subjects' macrophages. Both AD and normal macrophages were inhibited in αβ export across the blood-brain barrier due to adherence of Aβ-engorged macrophages to the endothelial layer. In comparison to normal subjects' macrophages, AD macrophages ingested and cleared less αβ, and underwent apoptosis upon exposure to soluble, protofibrillar, or fibrillar αβ. Confocal microscopy of stained AD brain sections revealed oligomeric Aβ in neurons and apoptotic macrophages, which surrounded and infiltrated congophilic microvessels, and fibrillar Aβ in plaques and microvessel walls. After incubation with AD brain sections, normal subjects' monocytes intruded into neurons and uploaded oligomeric Aβ. In conclusion, in patients with AD, macrophages appear to shuttle Aβ from neurons to vessels where their apoptosis may release fibrillar Aβ, contributing to cerebral amyloid angiopathy.
KW - Alzheimer
KW - Amyloid-beta
KW - Angiopathy
KW - Apoptosis
KW - Congophillic
KW - Macrophages
UR - http://www.scopus.com/inward/record.url?scp=59249103997&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=59249103997&partnerID=8YFLogxK
U2 - 10.1007/s00401-008-0481-0
DO - 10.1007/s00401-008-0481-0
M3 - Article
C2 - 19139910
AN - SCOPUS:59249103997
VL - 117
SP - 111
EP - 124
JO - Acta Neuropathologica
JF - Acta Neuropathologica
SN - 0001-6322
IS - 2
ER -