TY - JOUR
T1 - Alterations in kappa opioid receptor mRNA levels in the paraventricular nucleus of the hypothalamus by stress and sex steroids
AU - Yukhananov, Rustam Y.
AU - Handa, Robert J.
PY - 1996
Y1 - 1996
N2 - In this study, the levels of kappa opioid receptor (kOR) mRNA were determined using in situ hybridization following two types of stress (i.p. injection of hypertonic saline or novelty). In addition, we examined the possibility that estrogen or androgen would modify kOR mRNA. Gonadectomized male rats treated with estrogen or dihydrotestosterone were sacrificed 45 min after spending 15 min in a novel open field, or 60 min following hypertonic saline injection. Two-way ANOVA revealed that estrogen and novelty increased the levels of kOR mRNA in the ventral zone of the medial parvocellular part of the paraventricular nucleus (PVN), but not in the lateral parvocellular part of the PVN, claustrum, nucleus accumbens or the nucleus of the lateral olfactory tract. Furthermore, novelty increased kOR mRNA in gonadectomized (GDX) and GDX rats treated with dihydrotestosterone (DHTP), but not in sham-operated or estrogen-treated animals. Taken together, these data indicate that kOR mRNA levels are under estrogenic control and up-regulated in a stressor specific fashion.
AB - In this study, the levels of kappa opioid receptor (kOR) mRNA were determined using in situ hybridization following two types of stress (i.p. injection of hypertonic saline or novelty). In addition, we examined the possibility that estrogen or androgen would modify kOR mRNA. Gonadectomized male rats treated with estrogen or dihydrotestosterone were sacrificed 45 min after spending 15 min in a novel open field, or 60 min following hypertonic saline injection. Two-way ANOVA revealed that estrogen and novelty increased the levels of kOR mRNA in the ventral zone of the medial parvocellular part of the paraventricular nucleus (PVN), but not in the lateral parvocellular part of the PVN, claustrum, nucleus accumbens or the nucleus of the lateral olfactory tract. Furthermore, novelty increased kOR mRNA in gonadectomized (GDX) and GDX rats treated with dihydrotestosterone (DHTP), but not in sham-operated or estrogen-treated animals. Taken together, these data indicate that kOR mRNA levels are under estrogenic control and up-regulated in a stressor specific fashion.
KW - Androgen
KW - Estrogen
KW - Gonadal steroids
KW - Kappa opioid receptor mRNA
KW - Novelty
KW - PVN
KW - Stress: nucleus accumbens
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U2 - 10.1097/00001756-199607080-00033
DO - 10.1097/00001756-199607080-00033
M3 - Article
C2 - 8904783
AN - SCOPUS:0029780041
SN - 0959-4965
VL - 7
SP - 1690
EP - 1694
JO - NeuroReport
JF - NeuroReport
IS - 10
ER -