Alterations in intermediate filament proteins in rat kidney proximal tubule epithelial cells

Paul B. Hatzinger; Qin Chen; Liqun Dong; James L. Stevens

doi:10.1016/S0006-291X(88)81018-0

Alterations in intermediate filament proteins in rat kidney proximal tubule epithelial cells

Paul B. Hatzinger, Qin Chen, Liqun Dong, James L. Stevens

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Changes in the intermediate filament composition of rat kidney proximal tubule cells in culture have been investigated. The data suggest that differentiated tubular epithelial cells do not express vimentin, but vimentin expression is induced when the cells begin to proliferate in culture. The cultured cells are positive for both cytokeratins and vimentin by immunofluorescence microscopy. The data support the concept that the intermediate filament composition of proximal tubule epithelial cells can be altered during proliferation induced by nephrotoxic chemicals or by neoplastic transformation.

Original language	English (US)
Pages (from-to)	1316-1322
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	157
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(88)81018-0
State	Published - Dec 30 1988
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(88)81018-0

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title = "Alterations in intermediate filament proteins in rat kidney proximal tubule epithelial cells",

abstract = "Changes in the intermediate filament composition of rat kidney proximal tubule cells in culture have been investigated. The data suggest that differentiated tubular epithelial cells do not express vimentin, but vimentin expression is induced when the cells begin to proliferate in culture. The cultured cells are positive for both cytokeratins and vimentin by immunofluorescence microscopy. The data support the concept that the intermediate filament composition of proximal tubule epithelial cells can be altered during proliferation induced by nephrotoxic chemicals or by neoplastic transformation.",

author = "Hatzinger, {Paul B.} and Qin Chen and Liqun Dong and Stevens, {James L.}",

note = "Funding Information: The authors would like to thank Dr. Susan Jaken and Dr. Ryoichi Matsuda for assistance with the immunofluorescence experiments. We would also like to thank Dr. Thomas W. Jones for many helpful discussions. The work was supported by grants CA48197 from the National Cancer Institute and DK38925 from the National Institute of Diabetes, Digestive and Kidney Diseases to J.L.S.",

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doi = "10.1016/S0006-291X(88)81018-0",

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T1 - Alterations in intermediate filament proteins in rat kidney proximal tubule epithelial cells

AU - Hatzinger, Paul B.

AU - Chen, Qin

AU - Dong, Liqun

AU - Stevens, James L.

N1 - Funding Information: The authors would like to thank Dr. Susan Jaken and Dr. Ryoichi Matsuda for assistance with the immunofluorescence experiments. We would also like to thank Dr. Thomas W. Jones for many helpful discussions. The work was supported by grants CA48197 from the National Cancer Institute and DK38925 from the National Institute of Diabetes, Digestive and Kidney Diseases to J.L.S.

PY - 1988/12/30

Y1 - 1988/12/30

N2 - Changes in the intermediate filament composition of rat kidney proximal tubule cells in culture have been investigated. The data suggest that differentiated tubular epithelial cells do not express vimentin, but vimentin expression is induced when the cells begin to proliferate in culture. The cultured cells are positive for both cytokeratins and vimentin by immunofluorescence microscopy. The data support the concept that the intermediate filament composition of proximal tubule epithelial cells can be altered during proliferation induced by nephrotoxic chemicals or by neoplastic transformation.

AB - Changes in the intermediate filament composition of rat kidney proximal tubule cells in culture have been investigated. The data suggest that differentiated tubular epithelial cells do not express vimentin, but vimentin expression is induced when the cells begin to proliferate in culture. The cultured cells are positive for both cytokeratins and vimentin by immunofluorescence microscopy. The data support the concept that the intermediate filament composition of proximal tubule epithelial cells can be altered during proliferation induced by nephrotoxic chemicals or by neoplastic transformation.

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Alterations in intermediate filament proteins in rat kidney proximal tubule epithelial cells

Abstract

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