Alpha-2 adrenergic receptors stimulate actin organization in developing fetal rat cardiac myocytes

Amy C. Porter; Samuel P.S. Svensson; W. Daniel Stamer; Joseph J. Bahl; Jeremy G. Richman; John W. Regan

doi:10.1016/S0024-3205(02)02381-0

Alpha-2 adrenergic receptors stimulate actin organization in developing fetal rat cardiac myocytes

Amy C. Porter
, Samuel P.S. Svensson
, W. Daniel Stamer
, Joseph J. Bahl
, Jeremy G. Richman
, John W. Regan

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Expression of α₂-adrenergic receptors (α₂-AR) is very high in fetal rat heart although numbers decline with increasing gestational age. The current experiments were designed to identify the subtypes of α₂-AR expressed and the function of these receptors in fetal cardiac myocytes. Expression of α₂A and α₂C, but not α₂B, was confirmed in the myocyte population by indirect immunofluorescence microscopy with subtype-specific antibodies and by Western blot. Both dexmedetomidine, an α₂-selective agonist, and norepinephrine, increased actin cytoskeleton organization and this increase was blocked by the α₂-selective antagonist, atipamezole. Furthermore, dexmedetomidine inhibited isoproterenol-stimulated cAMP accumulation in isolated fetal rat heart and this was blocked by rauwolscine. Therefore, functional α₂A and α₂B subtypes are present in the fetal rat heart where they may have a role in cardiac development.

Original language	English (US)
Pages (from-to)	1455-1466
Number of pages	12
Journal	Life Sciences
Volume	72
Issue number	13
DOIs	https://doi.org/10.1016/S0024-3205(02)02381-0
State	Published - Feb 14 2003

Keywords

Actin
Cytoskeleton
Fetal heart
Immunocytochemistry
Phalloidin
α-adrenergic receptors

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Pharmacology, Toxicology and Pharmaceutics

Access to Document

10.1016/S0024-3205(02)02381-0

Cite this

@article{e08bddd2307f433893e537a1ab69e2fa,

title = "Alpha-2 adrenergic receptors stimulate actin organization in developing fetal rat cardiac myocytes",

abstract = "Expression of α2-adrenergic receptors (α2-AR) is very high in fetal rat heart although numbers decline with increasing gestational age. The current experiments were designed to identify the subtypes of α2-AR expressed and the function of these receptors in fetal cardiac myocytes. Expression of α2A and α2C, but not α2B, was confirmed in the myocyte population by indirect immunofluorescence microscopy with subtype-specific antibodies and by Western blot. Both dexmedetomidine, an α2-selective agonist, and norepinephrine, increased actin cytoskeleton organization and this increase was blocked by the α2-selective antagonist, atipamezole. Furthermore, dexmedetomidine inhibited isoproterenol-stimulated cAMP accumulation in isolated fetal rat heart and this was blocked by rauwolscine. Therefore, functional α2A and α2B subtypes are present in the fetal rat heart where they may have a role in cardiac development.",

keywords = "Actin, Cytoskeleton, Fetal heart, Immunocytochemistry, Phalloidin, α-adrenergic receptors",

author = "Porter, \{Amy C.\} and Svensson, \{Samuel P.S.\} and Stamer, \{W. Daniel\} and Bahl, \{Joseph J.\} and Richman, \{Jeremy G.\} and Regan, \{John W.\}",

note = "Funding Information: This work has been supported by grants from the Arizona Affiliate of the American Heart Association (AZ-94-GS-37) and by the R. W. Johnson Pharmaceutical Research Institute. Additional support has been provided to J.W.R. from the National Institutes of Health (EY09355) and by Allergan Inc. S. P. S. Svensson is supported by a postdoctoral fellowship from the Swedish Society for Medical Research, The Swedish Medical Research Council and Sandoz. J.J.B. is supported by the National Institutes of Health (HL20984) and the University Heart Center. We would like to thank Dr. J. M. Savola (Orion Corp./Farmos) for his interest in this work. We would also like to thank Yewen Wu for his expert preparation of primary cultures of fetal cardiac myocytes.",

year = "2003",

month = feb,

day = "14",

doi = "10.1016/S0024-3205(02)02381-0",

language = "English (US)",

volume = "72",

pages = "1455--1466",

journal = "Life Sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

number = "13",

}

TY - JOUR

T1 - Alpha-2 adrenergic receptors stimulate actin organization in developing fetal rat cardiac myocytes

AU - Porter, Amy C.

AU - Svensson, Samuel P.S.

AU - Stamer, W. Daniel

AU - Bahl, Joseph J.

AU - Richman, Jeremy G.

AU - Regan, John W.

N1 - Funding Information: This work has been supported by grants from the Arizona Affiliate of the American Heart Association (AZ-94-GS-37) and by the R. W. Johnson Pharmaceutical Research Institute. Additional support has been provided to J.W.R. from the National Institutes of Health (EY09355) and by Allergan Inc. S. P. S. Svensson is supported by a postdoctoral fellowship from the Swedish Society for Medical Research, The Swedish Medical Research Council and Sandoz. J.J.B. is supported by the National Institutes of Health (HL20984) and the University Heart Center. We would like to thank Dr. J. M. Savola (Orion Corp./Farmos) for his interest in this work. We would also like to thank Yewen Wu for his expert preparation of primary cultures of fetal cardiac myocytes.

PY - 2003/2/14

Y1 - 2003/2/14

N2 - Expression of α2-adrenergic receptors (α2-AR) is very high in fetal rat heart although numbers decline with increasing gestational age. The current experiments were designed to identify the subtypes of α2-AR expressed and the function of these receptors in fetal cardiac myocytes. Expression of α2A and α2C, but not α2B, was confirmed in the myocyte population by indirect immunofluorescence microscopy with subtype-specific antibodies and by Western blot. Both dexmedetomidine, an α2-selective agonist, and norepinephrine, increased actin cytoskeleton organization and this increase was blocked by the α2-selective antagonist, atipamezole. Furthermore, dexmedetomidine inhibited isoproterenol-stimulated cAMP accumulation in isolated fetal rat heart and this was blocked by rauwolscine. Therefore, functional α2A and α2B subtypes are present in the fetal rat heart where they may have a role in cardiac development.

AB - Expression of α2-adrenergic receptors (α2-AR) is very high in fetal rat heart although numbers decline with increasing gestational age. The current experiments were designed to identify the subtypes of α2-AR expressed and the function of these receptors in fetal cardiac myocytes. Expression of α2A and α2C, but not α2B, was confirmed in the myocyte population by indirect immunofluorescence microscopy with subtype-specific antibodies and by Western blot. Both dexmedetomidine, an α2-selective agonist, and norepinephrine, increased actin cytoskeleton organization and this increase was blocked by the α2-selective antagonist, atipamezole. Furthermore, dexmedetomidine inhibited isoproterenol-stimulated cAMP accumulation in isolated fetal rat heart and this was blocked by rauwolscine. Therefore, functional α2A and α2B subtypes are present in the fetal rat heart where they may have a role in cardiac development.

KW - Actin

KW - Cytoskeleton

KW - Fetal heart

KW - Immunocytochemistry

KW - Phalloidin

KW - α-adrenergic receptors

UR - https://www.scopus.com/pages/publications/0037435808

UR - https://www.scopus.com/pages/publications/0037435808#tab=citedBy

U2 - 10.1016/S0024-3205(02)02381-0

DO - 10.1016/S0024-3205(02)02381-0

M3 - Article

C2 - 12535714

AN - SCOPUS:0037435808

SN - 0024-3205

VL - 72

SP - 1455

EP - 1466

JO - Life Sciences

JF - Life Sciences

IS - 13

ER -

Alpha-2 adrenergic receptors stimulate actin organization in developing fetal rat cardiac myocytes

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this