All-reflective multi-modal nonlinear microscopy with minimal alignment

Benjamin Cromey, Yukun Qin, Orkhongua Batjargal, Joshua Magnus, Sean Crystal, Khanh Kieu

Research output: Chapter in Book/Report/Conference proceedingConference contribution


The design of an all-reflective laser-scanning microscope capable of imaging samples three-dimensionally with multiple modes of nonlinear imaging at high resolution and with a large field of view is described in this presentation. The all-reflective design is based on off-axis parabolic mirror sections, and was designed to require minimal alignment. An all fiber alignment-free femtosecond laser with dual output is used to enable simultaneous multiphoton and stimulated Raman imaging processes, creating a powerful but hassle-free system. This paper will describe the design, performance, and imaging outputs of the system.

Original languageEnglish (US)
Title of host publicationMultimodal Biomedical Imaging XVII
EditorsFred S. Azar, Xavier Intes, Qianqian Fang
ISBN (Electronic)9781510647756
StatePublished - 2022
EventMultimodal Biomedical Imaging XVII 2022 - San Francisco, United States
Duration: Jan 22 2022Jan 27 2022

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceMultimodal Biomedical Imaging XVII 2022
Country/TerritoryUnited States
CitySan Francisco


  • Fiber laser
  • Microscopy
  • Multimodal
  • Multiphoton
  • Optical design
  • Raman

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging


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