Alfalfa vein mottling virus, a novel potyvirid infecting Medicago sativa L.

Lev G. Nemchinov, Olga A. Postnikova, William M. Wintermantel, John C. Palumbo, Sam Grinstead

Research output: Contribution to journalArticlepeer-review

Abstract

Background: We have recently identified a novel virus detected in alfalfa seed material. The virus was tentatively named alfalfa-associated potyvirus 1, as its genomic fragments bore similarities with potyvirids. In this study, we continued investigating this novel species, expanding information on its genomic features and biological characteristics. Methods: This research used a wide range of methodology to achieve end results: high throughput sequencing, bioinformatics tools, reverse transcription-polymerase chain reactions, differential diagnostics using indicator plants, virus purification, transmission electron microscopy, and others. Results: In this study, we obtained a complete genome sequence of the virus and classified it as a tentative species in the new genus, most closely related to the members of the genus Ipomovirus in the family Potyviridae. This assumption is based on the genome sequence and structure, phylogenetic relationships, and transmission electron microscopy investigations. We also demonstrated its mechanical transmission to the indicator plant Nicotiana benthamiana and to the natural host Medicago sativa, both of which developed characteristic symptoms therefore suggesting a pathogenic nature of the disease. Conclusions: Consistent with symptomatology, the virus was renamed to alfalfa vein mottling virus. A name Alvemovirus was proposed for the new genus in the family Potyviridae, of which alfalfa vein mottling virus is a tentative member.

Original languageEnglish (US)
Article number284
JournalVirology Journal
Volume20
Issue number1
DOIs
StatePublished - Dec 2023
Externally publishedYes

Keywords

  • Alfalfa (Medicago sativa L.)
  • Alfalfa vein mottling virus
  • Alvemovirus
  • Plant viruses
  • Positive-strand RNA viruses
  • Potyviridae

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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