Ah receptor-independent induction of CYP1A2 gene expression in genetically inbred mice

Mona Gupta; John Miggens; Alan Parrish; James Womack; Kenneth S. Ramos; Louis V. Rodriguez; Lawrence S. Goldstein; Carol Holtzapple; Larry Stanker; Stephen H. Safe

doi:10.1016/S1382-6689(98)00004-0

Ah receptor-independent induction of CYP1A2 gene expression in genetically inbred mice

Mona Gupta, John Miggens, Alan Parrish, James Womack, Kenneth S. Ramos, Louis V. Rodriguez, Lawrence S. Goldstein, Carol Holtzapple, Larry Stanker, Stephen H. Safe

Medicine

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Acenaphthylene is a tricyclic aromatic hydrocarbon which induces hepatic methoxyresorufin O-demethylase (MROD) activity and CYP1A2 mRNA levels in 2 week-old male B6C3F1 mice. In the present study, this induction response was further investigated in genetically-inbred mice which differ in their aryl- hydrocarbon (Ah)-responsiveness. Acenapthylene (300 mg/kg) induced a 5- to 23-fold induction of MROD activity in Ah-nonresponsive (DBA and SJL) and responsive (C3H, C57/BL6, A/J, CBA and B6C3F1) mice. The highest induction response was observed in the DBA strain in which there was a 23- and 15-fold increase in activity in males and females, respectively. Acenaphthylene also caused a 2-fold increase in CYP1A2 mRNA and immunoreactive protein levels in 2 week-old DBA mice; however, this induction response was not observed in 6 week-old animals. For example, MROD activity in 6 week-old DBA mice was induced <2-fold by acenaphthylene, mainly as a consequence of increased basal CYP1A2 expression and MROD activity which, at the age of 6 weeks, approached levels induced by acenaphthylene in the 2 week-old mice. This was also observed by immunohistochemical staining with CYP1A2 antibodies of 2 and 6 week-old hepatic tissue from treated and control mice which also showed that CYP1A2 induction was dependent on the age of the animals.

Original language	English (US)
Pages (from-to)	205-213
Number of pages	9
Journal	Environmental Toxicology and Pharmacology
Volume	5
Issue number	3
DOIs	https://doi.org/10.1016/S1382-6689(98)00004-0
State	Published - May 1998

Keywords

Acenaphthylene
Ah receptor-independent
CYP1A2 induction
Mice

ASJC Scopus subject areas

Toxicology
Pharmacology
Health, Toxicology and Mutagenesis

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10.1016/S1382-6689(98)00004-0

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@article{66f9d99f4c19402db38c63e30527dc2e,

title = "Ah receptor-independent induction of CYP1A2 gene expression in genetically inbred mice",

abstract = "Acenaphthylene is a tricyclic aromatic hydrocarbon which induces hepatic methoxyresorufin O-demethylase (MROD) activity and CYP1A2 mRNA levels in 2 week-old male B6C3F1 mice. In the present study, this induction response was further investigated in genetically-inbred mice which differ in their aryl- hydrocarbon (Ah)-responsiveness. Acenapthylene (300 mg/kg) induced a 5- to 23-fold induction of MROD activity in Ah-nonresponsive (DBA and SJL) and responsive (C3H, C57/BL6, A/J, CBA and B6C3F1) mice. The highest induction response was observed in the DBA strain in which there was a 23- and 15-fold increase in activity in males and females, respectively. Acenaphthylene also caused a 2-fold increase in CYP1A2 mRNA and immunoreactive protein levels in 2 week-old DBA mice; however, this induction response was not observed in 6 week-old animals. For example, MROD activity in 6 week-old DBA mice was induced <2-fold by acenaphthylene, mainly as a consequence of increased basal CYP1A2 expression and MROD activity which, at the age of 6 weeks, approached levels induced by acenaphthylene in the 2 week-old mice. This was also observed by immunohistochemical staining with CYP1A2 antibodies of 2 and 6 week-old hepatic tissue from treated and control mice which also showed that CYP1A2 induction was dependent on the age of the animals.",

keywords = "Acenaphthylene, Ah receptor-independent, CYP1A2 induction, Mice",

author = "Mona Gupta and John Miggens and Alan Parrish and James Womack and Ramos, {Kenneth S.} and Rodriguez, {Louis V.} and Goldstein, {Lawrence S.} and Carol Holtzapple and Larry Stanker and Safe, {Stephen H.}",

note = "Funding Information: The financial assistance of the Electric Power Research Institute (contract WO-2963-04), the National Institutes of Health (ES04917), and the Texas Agricultural Experiment Station is gratefully acknowledged.",

year = "1998",

month = may,

doi = "10.1016/S1382-6689(98)00004-0",

language = "English (US)",

volume = "5",

pages = "205--213",

journal = "Environmental Toxicology and Pharmacology",

issn = "1382-6689",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - Ah receptor-independent induction of CYP1A2 gene expression in genetically inbred mice

AU - Gupta, Mona

AU - Miggens, John

AU - Parrish, Alan

AU - Womack, James

AU - Ramos, Kenneth S.

AU - Rodriguez, Louis V.

AU - Goldstein, Lawrence S.

AU - Holtzapple, Carol

AU - Stanker, Larry

AU - Safe, Stephen H.

N1 - Funding Information: The financial assistance of the Electric Power Research Institute (contract WO-2963-04), the National Institutes of Health (ES04917), and the Texas Agricultural Experiment Station is gratefully acknowledged.

PY - 1998/5

Y1 - 1998/5

N2 - Acenaphthylene is a tricyclic aromatic hydrocarbon which induces hepatic methoxyresorufin O-demethylase (MROD) activity and CYP1A2 mRNA levels in 2 week-old male B6C3F1 mice. In the present study, this induction response was further investigated in genetically-inbred mice which differ in their aryl- hydrocarbon (Ah)-responsiveness. Acenapthylene (300 mg/kg) induced a 5- to 23-fold induction of MROD activity in Ah-nonresponsive (DBA and SJL) and responsive (C3H, C57/BL6, A/J, CBA and B6C3F1) mice. The highest induction response was observed in the DBA strain in which there was a 23- and 15-fold increase in activity in males and females, respectively. Acenaphthylene also caused a 2-fold increase in CYP1A2 mRNA and immunoreactive protein levels in 2 week-old DBA mice; however, this induction response was not observed in 6 week-old animals. For example, MROD activity in 6 week-old DBA mice was induced <2-fold by acenaphthylene, mainly as a consequence of increased basal CYP1A2 expression and MROD activity which, at the age of 6 weeks, approached levels induced by acenaphthylene in the 2 week-old mice. This was also observed by immunohistochemical staining with CYP1A2 antibodies of 2 and 6 week-old hepatic tissue from treated and control mice which also showed that CYP1A2 induction was dependent on the age of the animals.

AB - Acenaphthylene is a tricyclic aromatic hydrocarbon which induces hepatic methoxyresorufin O-demethylase (MROD) activity and CYP1A2 mRNA levels in 2 week-old male B6C3F1 mice. In the present study, this induction response was further investigated in genetically-inbred mice which differ in their aryl- hydrocarbon (Ah)-responsiveness. Acenapthylene (300 mg/kg) induced a 5- to 23-fold induction of MROD activity in Ah-nonresponsive (DBA and SJL) and responsive (C3H, C57/BL6, A/J, CBA and B6C3F1) mice. The highest induction response was observed in the DBA strain in which there was a 23- and 15-fold increase in activity in males and females, respectively. Acenaphthylene also caused a 2-fold increase in CYP1A2 mRNA and immunoreactive protein levels in 2 week-old DBA mice; however, this induction response was not observed in 6 week-old animals. For example, MROD activity in 6 week-old DBA mice was induced <2-fold by acenaphthylene, mainly as a consequence of increased basal CYP1A2 expression and MROD activity which, at the age of 6 weeks, approached levels induced by acenaphthylene in the 2 week-old mice. This was also observed by immunohistochemical staining with CYP1A2 antibodies of 2 and 6 week-old hepatic tissue from treated and control mice which also showed that CYP1A2 induction was dependent on the age of the animals.

KW - Acenaphthylene

KW - Ah receptor-independent

KW - CYP1A2 induction

KW - Mice

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U2 - 10.1016/S1382-6689(98)00004-0

DO - 10.1016/S1382-6689(98)00004-0

M3 - Article

C2 - 21781866

AN - SCOPUS:0031835388

SN - 1382-6689

VL - 5

SP - 205

EP - 213

JO - Environmental Toxicology and Pharmacology

JF - Environmental Toxicology and Pharmacology

IS - 3

ER -

Ah receptor-independent induction of CYP1A2 gene expression in genetically inbred mice

Abstract

Keywords

ASJC Scopus subject areas

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