TY - JOUR
T1 - Aggregated Platelets Enhance Adherence of Candida Yeasts to Endothelium
AU - Klotz, Stephen A.
AU - Harrison, Jeffery L.
AU - Misra, Raghunath P.
N1 - Funding Information:
Received for publication 14 October 1988 and in revised form 12 May 1989. This work was supported by Veterans Administration Research Funds. The authors thank Ann Shows and Charles E. Self for assistance. Please address requests for reprints to Dr. Stephen A. Klotz, Infectious Disease Section (111 10), VA Medical Center, 510 East Stoner Avenue, Shreveport, LA 71101-4295.
PY - 1989/10
Y1 - 1989/10
N2 - The adherence of Candida albicans yeasts to human umbilical vein endothelium and to subendothelial extracellular matrix (ECM) was investigated. Yeasts added to confluent endothelium in titrated platelet-poor plasma adhered on the average of 1 colony forming unit (cfu) per culture well. When platelets were added as platelet-rich plasma, a significant increase of yeast adherence was not seen. However, when endothelium was contracted by treatment with 2 mA/EDTA, resulting in exposure of ECM, yeast adherence was increased to 10 cfu/well. When platelets were added with these yeasts, the number of adhering yeasts was further increased to 23 cfu/well (P <.01). This represented an increase in adherence of yeasts of 230%. When the endothelial cells were completely removed and ECM exposed, platelets were found to likewise augment yeast adherence. Platelets, when added to the ECM, formed aggregates to which the yeasts firmly adhered. Likewise, when platelets were aggregated by adenosine diphosphate and mixed with yeasts, yeasts were shown to bind avidly to aggregated platelets, whereas yeasts did not adhere to unactivated, discoid platelets. Thus, exposed subendothelial ECM induces the aggregation of platelets and yeasts bind avidly to these platelet aggregates.
AB - The adherence of Candida albicans yeasts to human umbilical vein endothelium and to subendothelial extracellular matrix (ECM) was investigated. Yeasts added to confluent endothelium in titrated platelet-poor plasma adhered on the average of 1 colony forming unit (cfu) per culture well. When platelets were added as platelet-rich plasma, a significant increase of yeast adherence was not seen. However, when endothelium was contracted by treatment with 2 mA/EDTA, resulting in exposure of ECM, yeast adherence was increased to 10 cfu/well. When platelets were added with these yeasts, the number of adhering yeasts was further increased to 23 cfu/well (P <.01). This represented an increase in adherence of yeasts of 230%. When the endothelial cells were completely removed and ECM exposed, platelets were found to likewise augment yeast adherence. Platelets, when added to the ECM, formed aggregates to which the yeasts firmly adhered. Likewise, when platelets were aggregated by adenosine diphosphate and mixed with yeasts, yeasts were shown to bind avidly to aggregated platelets, whereas yeasts did not adhere to unactivated, discoid platelets. Thus, exposed subendothelial ECM induces the aggregation of platelets and yeasts bind avidly to these platelet aggregates.
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U2 - 10.1093/infdis/160.4.669
DO - 10.1093/infdis/160.4.669
M3 - Article
C2 - 2677163
AN - SCOPUS:0024421469
SN - 0022-1899
VL - 160
SP - 669
EP - 677
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -