Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)2 vitamin D3

Hua Xu; Liqun Bai; James F. Collins; Fayez K. Ghishan

doi:10.1152/ajpcell.00412.2001

Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)₂ vitamin D₃

Hua Xu
, Liqun Bai
, James F. Collins
, Fayez K. Ghishan

Research output: Contribution to journal › Article › peer-review

194 Scopus citations

Abstract

The current studies were designed to characterize type IIb sodium-inorganic phosphate (P_i) cotransporter (NaP_i-IIb) expression and to assess the effect of 1,25-(OH)₂ vitamin D₃ on NaP_i-IIb gene expression during rat ontogeny. Sodium-dependent P_i absorption by intestinal brush-border membrane vesicles (BBMVs) decreased with age, and NaP_i-IIb gene expression also decreased proportionally with age. 1,25-(OH)₂ vitamin D₃ treatment increased intestinal BBMV P_i absorption by ∼2.5-fold in suckling rats and by ∼2.1-fold in adult rats. 1,25-(OH)₂ vitamin D₃ treatment also increased NaP_iIIb mRNA abundance by ∼2-fold in 14-day-old rats but had no effect on mRNA expression in adults. Furthermore, in rat intestinal epithelial (RIE) cells, 1,25-(OH)₂ vitamin D₃ increased NaP_i-IIb mRNA abundance, an effect that was abolished by actinomycin D. Additionally, human NaP_i-IIb gene promoter activity in transiently transfected RIE cells showed ∼1.6-fold increase after 1,25-(OH)₂ vitamin D₃ treatment. In conclusion, we demonstrate that the age-related decrease in intestinal sodium-dependent P_i absorption correlates with decreased NaP_i-IIb mRNA expression. Our data also suggest that the effect of 1,25-(OH)₂ vitamin D₃ on NaP_i-IIb expression is at least partially mediated by gene transcription in suckling rats.

Original language	English (US)
Pages (from-to)	C487-C493
Journal	American Journal of Physiology - Cell Physiology
Volume	282
Issue number	3 51-3
DOIs	https://doi.org/10.1152/ajpcell.00412.2001
State	Published - 2002

Keywords

Gene regulation
Ontogeny
Rat intestinal epithelial cells

ASJC Scopus subject areas

Physiology
Cell Biology

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10.1152/ajpcell.00412.2001

Cite this

@article{184396ff69994256b221993f01f26916,

title = "Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)2 vitamin D3",

abstract = "The current studies were designed to characterize type IIb sodium-inorganic phosphate (Pi) cotransporter (NaPi-IIb) expression and to assess the effect of 1,25-(OH)2 vitamin D3 on NaPi-IIb gene expression during rat ontogeny. Sodium-dependent Pi absorption by intestinal brush-border membrane vesicles (BBMVs) decreased with age, and NaPi-IIb gene expression also decreased proportionally with age. 1,25-(OH)2 vitamin D3 treatment increased intestinal BBMV Pi absorption by ∼2.5-fold in suckling rats and by ∼2.1-fold in adult rats. 1,25-(OH)2 vitamin D3 treatment also increased NaPiIIb mRNA abundance by ∼2-fold in 14-day-old rats but had no effect on mRNA expression in adults. Furthermore, in rat intestinal epithelial (RIE) cells, 1,25-(OH)2 vitamin D3 increased NaPi-IIb mRNA abundance, an effect that was abolished by actinomycin D. Additionally, human NaPi-IIb gene promoter activity in transiently transfected RIE cells showed ∼1.6-fold increase after 1,25-(OH)2 vitamin D3 treatment. In conclusion, we demonstrate that the age-related decrease in intestinal sodium-dependent Pi absorption correlates with decreased NaPi-IIb mRNA expression. Our data also suggest that the effect of 1,25-(OH)2 vitamin D3 on NaPi-IIb expression is at least partially mediated by gene transcription in suckling rats.",

keywords = "Gene regulation, Ontogeny, Rat intestinal epithelial cells",

author = "Hua Xu and Liqun Bai and Collins, \{James F.\} and Ghishan, \{Fayez K.\}",

year = "2002",

doi = "10.1152/ajpcell.00412.2001",

language = "English (US)",

volume = "282",

pages = "C487--C493",

journal = "American Journal of Physiology - Cell Physiology",

issn = "0363-6143",

publisher = "American Physiological Society",

number = "3 51-3",

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T1 - Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)2 vitamin D3

AU - Xu, Hua

AU - Bai, Liqun

AU - Collins, James F.

AU - Ghishan, Fayez K.

PY - 2002

Y1 - 2002

N2 - The current studies were designed to characterize type IIb sodium-inorganic phosphate (Pi) cotransporter (NaPi-IIb) expression and to assess the effect of 1,25-(OH)2 vitamin D3 on NaPi-IIb gene expression during rat ontogeny. Sodium-dependent Pi absorption by intestinal brush-border membrane vesicles (BBMVs) decreased with age, and NaPi-IIb gene expression also decreased proportionally with age. 1,25-(OH)2 vitamin D3 treatment increased intestinal BBMV Pi absorption by ∼2.5-fold in suckling rats and by ∼2.1-fold in adult rats. 1,25-(OH)2 vitamin D3 treatment also increased NaPiIIb mRNA abundance by ∼2-fold in 14-day-old rats but had no effect on mRNA expression in adults. Furthermore, in rat intestinal epithelial (RIE) cells, 1,25-(OH)2 vitamin D3 increased NaPi-IIb mRNA abundance, an effect that was abolished by actinomycin D. Additionally, human NaPi-IIb gene promoter activity in transiently transfected RIE cells showed ∼1.6-fold increase after 1,25-(OH)2 vitamin D3 treatment. In conclusion, we demonstrate that the age-related decrease in intestinal sodium-dependent Pi absorption correlates with decreased NaPi-IIb mRNA expression. Our data also suggest that the effect of 1,25-(OH)2 vitamin D3 on NaPi-IIb expression is at least partially mediated by gene transcription in suckling rats.

AB - The current studies were designed to characterize type IIb sodium-inorganic phosphate (Pi) cotransporter (NaPi-IIb) expression and to assess the effect of 1,25-(OH)2 vitamin D3 on NaPi-IIb gene expression during rat ontogeny. Sodium-dependent Pi absorption by intestinal brush-border membrane vesicles (BBMVs) decreased with age, and NaPi-IIb gene expression also decreased proportionally with age. 1,25-(OH)2 vitamin D3 treatment increased intestinal BBMV Pi absorption by ∼2.5-fold in suckling rats and by ∼2.1-fold in adult rats. 1,25-(OH)2 vitamin D3 treatment also increased NaPiIIb mRNA abundance by ∼2-fold in 14-day-old rats but had no effect on mRNA expression in adults. Furthermore, in rat intestinal epithelial (RIE) cells, 1,25-(OH)2 vitamin D3 increased NaPi-IIb mRNA abundance, an effect that was abolished by actinomycin D. Additionally, human NaPi-IIb gene promoter activity in transiently transfected RIE cells showed ∼1.6-fold increase after 1,25-(OH)2 vitamin D3 treatment. In conclusion, we demonstrate that the age-related decrease in intestinal sodium-dependent Pi absorption correlates with decreased NaPi-IIb mRNA expression. Our data also suggest that the effect of 1,25-(OH)2 vitamin D3 on NaPi-IIb expression is at least partially mediated by gene transcription in suckling rats.

KW - Gene regulation

KW - Ontogeny

KW - Rat intestinal epithelial cells

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