Agarose isoelectrofocusing of intact virions

Katherine S. Zerda, Charles P. Gerba

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


A convenient and accurate method for determining the isoelectric points of intact virions is described. Tritium-labeled poliovirus 1 (strains Brunhilde and LSc-2) and echovirus 1 (isolates V239, V248, V212, R115 and 4CH-1) were successfully focused into sharp bands at their respective isoelectric points using a thin-layer agarose isoelectric focusing system. In situ detection of labeled virus bands in the agarose was by fluorography. Freezing and thawing of virus samples prior to isoelectric focusing did not alter their respective isoelectric points.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalJournal of Virological Methods
Issue number1
StatePublished - Aug 1984


  • agarose IEF
  • isoelectric focusing
  • isoelectric points
  • virion pI

ASJC Scopus subject areas

  • Virology


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