Affinity of extracellular phosphatases for ELF97 phosphate in aquatic environments

Jiří Nedoma, France Van Wambeke, Alena Štrojsová, Martina Štrojsová, Solange Duhamel

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Recently, the phosphatase substrate ELF97 phosphate (ELFP) has been employed to study the presence of extracellular phosphatases in different plankton populations in natural aquatic environments. Kinetic properties of ELFP hydrolysis by natural extracellular phosphatases are, however, mostly unknown. We indirectly studied the affinity of extracellular phosphatases for ELFP in different aquatic environments through its ability to inhibit the hydrolysis of 4-methylumbelliferyl phosphate (4MUP). Values of inhibition constants, K i, which correspond to the concentrations necessary for half saturation of phosphatases by ELFP, were lowest (0.18-4.5 μmol L -1) in the oligotrophic Mediterranean Sea. We found higher values (i.e. lower affinity) in oligo- to mesotrophic acidified lakes (5.2-14 μmol L-1), in a eutrophic reservoir (13-35 μmol L-1) and in a pure culture of the marine bacterium Alteromonas infernus (29 μmol L -1). ELFP had a pronounced effect on the parameter KM (Michaelis constant) of 4MUP saturation kinetics, while its effect on the parameter Vmax was low. This behaviour is compatible with the assumption of competitive interaction between 4MUP and ELFP. Our experiments indicated that the assay ELFP concentration in the detection kit used was 250-500 μmol L-1 (after the recommended dilution to a ratio of 1:20), which would ensure >99% saturation of extracellular phosphatases in marine environments and >90% saturation in the studied fresh waters.

Original languageEnglish (US)
Pages (from-to)454-460
Number of pages7
JournalMarine and Freshwater Research
Issue number5
StatePublished - 2007
Externally publishedYes


  • Freshwater
  • Marine

ASJC Scopus subject areas

  • Oceanography
  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Ecology


Dive into the research topics of 'Affinity of extracellular phosphatases for ELF97 phosphate in aquatic environments'. Together they form a unique fingerprint.

Cite this