TY - JOUR
T1 - Adenosine and divalent cation effects on S-91 melanoma adenylate cyclase
AU - Bregman, Marvin D.
AU - Sawyer, Tomi K.
AU - Hadley, Mac E.
AU - Hruby, Victor J.
N1 - Funding Information:
I This work was supported, in part, by NSF Grant PCM 7’7-07031 and USPHS Grants AM 17420, AM 21085, and CA 200547. ’ Abbreviations used: Adenine-9-P-Araf, adenine-9-P-D-arabinofuranoside; MSH, melanocyte-stimulating hormone; Me, metal; [Nle’la-MSH, [norleucine4]-a-melanocyte-stimulating hormone; PGE, prostaglandin.
PY - 1980/4/15
Y1 - 1980/4/15
N2 - Adenosine inhibits S-91 melanoma membrane adenylate cyclase. This inhibition is observed with all activators tested; α-melanocyte-stimulating hormone, [norleucine4]α-melanocyte-stimulating hormone, prostaglandin-E2, and fluoride ion. This inhibition is also observed with the ribose modified adenosine analog adenine-9-β-d-arabinofuranoside. However, "R" site (stringent for the ribose moiety) analogs such as 2-chloroadenosine and 6-methyladenosine were without effect. Addition of manganous ion (Mn2+), a potent activator of adenylate cyclase, markedly enhances the inhibition by adenosine. The nucleoside specificity, Mn2+ requirement, and lack of reversal by the potent R-site antagonist, 3-isobutyl-1-methylxanthine, suggest that the S-91 melanoma membrane adenylate cyclase system contains only P-type (stringent for purine moiety) receptor sites.
AB - Adenosine inhibits S-91 melanoma membrane adenylate cyclase. This inhibition is observed with all activators tested; α-melanocyte-stimulating hormone, [norleucine4]α-melanocyte-stimulating hormone, prostaglandin-E2, and fluoride ion. This inhibition is also observed with the ribose modified adenosine analog adenine-9-β-d-arabinofuranoside. However, "R" site (stringent for the ribose moiety) analogs such as 2-chloroadenosine and 6-methyladenosine were without effect. Addition of manganous ion (Mn2+), a potent activator of adenylate cyclase, markedly enhances the inhibition by adenosine. The nucleoside specificity, Mn2+ requirement, and lack of reversal by the potent R-site antagonist, 3-isobutyl-1-methylxanthine, suggest that the S-91 melanoma membrane adenylate cyclase system contains only P-type (stringent for purine moiety) receptor sites.
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U2 - 10.1016/0003-9861(80)90480-4
DO - 10.1016/0003-9861(80)90480-4
M3 - Article
C2 - 7396490
AN - SCOPUS:0019158235
SN - 0003-9861
VL - 201
SP - 1
EP - 7
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 1
ER -