TY - JOUR
T1 - Acute changes in sputum IL-10 following underground exposure to diesel exhaust
AU - Burgess, Jefferey L.
AU - Fleming, Joy E.
AU - Mulenga, Emmanuel M.
AU - Josyula, Arun
AU - Hysong, Tracy A.
AU - Joggerst, Philip J.
AU - Kurzius-Spencer, Margaret
AU - Miller, Hugh B.
N1 - Funding Information:
This research and publication was supported by Training Grant No. T42/CCT918726 from the Centers for Disease Control and Prevention/ National Institute for Occupational Safety and Health and the National Institute for Environmental Health Sciences, Center Grant No. ES06694. The contents are solely the responsibility of the authors and do not necessarily represent the official views of the National Institute for Occupational Safety and Health.
PY - 2007/3
Y1 - 2007/3
N2 - Introduction. Although exposure to diesel exhaust has been linked with adverse health effects, little is known about the acute effects of exposure in the underground workplace. Methods. Cross-shift spirometry and sputum induction were completed on twelve subjects associated with comminuted rock removal (mucking) operations in an underground copper mine using diesel powered and pneumatic equipment on separate days, and sputum collected on a baseline non-exposure day as well. Results. For diesel operations, elemental carbon exposure averaged 538 ± 512 μg/m3 during the 1-2 hour operations. Sputum interleukin-10 decreased with diesel exhaust using one ELISA assay (3.69 v. 2.32 pg/ml, p = 0.015), but increased when measured with a different ELISA kit (0.18 v. 0.59 pg/ml, p = 0.019), consistent with an overall decline in IL-10 protein concentration but an increase in the biologically active form. Sputum interleukin-6 decreased with exposure to diesel exhaust, although this change lost statistical significance when restricted to non-smokers. There were no significant changes in spirometry, interleukins 1β, 4, and 8, tumor necrosis factor alpha or 8-hydroxy-2′-deoxyguanosine. Conclusion. High levels of diesel exhaust can result in rapid changes in sputum IL-10, suggesting possible protein modification.
AB - Introduction. Although exposure to diesel exhaust has been linked with adverse health effects, little is known about the acute effects of exposure in the underground workplace. Methods. Cross-shift spirometry and sputum induction were completed on twelve subjects associated with comminuted rock removal (mucking) operations in an underground copper mine using diesel powered and pneumatic equipment on separate days, and sputum collected on a baseline non-exposure day as well. Results. For diesel operations, elemental carbon exposure averaged 538 ± 512 μg/m3 during the 1-2 hour operations. Sputum interleukin-10 decreased with diesel exhaust using one ELISA assay (3.69 v. 2.32 pg/ml, p = 0.015), but increased when measured with a different ELISA kit (0.18 v. 0.59 pg/ml, p = 0.019), consistent with an overall decline in IL-10 protein concentration but an increase in the biologically active form. Sputum interleukin-6 decreased with exposure to diesel exhaust, although this change lost statistical significance when restricted to non-smokers. There were no significant changes in spirometry, interleukins 1β, 4, and 8, tumor necrosis factor alpha or 8-hydroxy-2′-deoxyguanosine. Conclusion. High levels of diesel exhaust can result in rapid changes in sputum IL-10, suggesting possible protein modification.
KW - Diesel exhaust
KW - Interleukin 10
KW - Mining
KW - Sputum
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U2 - 10.1080/15563650601072142
DO - 10.1080/15563650601072142
M3 - Article
C2 - 17453876
AN - SCOPUS:34047094989
SN - 1556-3650
VL - 45
SP - 255
EP - 260
JO - Clinical Toxicology
JF - Clinical Toxicology
IS - 3
ER -