Acidophilic degradation of methanol by a methanogenic enrichment culture

L. Florencio, A. Nozhevnikova, A. van Langerak, A. J.M. Stams, J. A. Field, G. Lettinga

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


An acidophilic methanogenic enrichment culture was obtained in a continuous up-flow anaerobic sludge blanket reactor operated at pH 4.2 with methanol as the sole carbon source. The specific methylotrophic methanogenic activity of the enriched reactor sludge at pH 5 was 3.57 g COD g-1 volatile suspended solids day-1 and the apparent doubling time of the biomass was 15.8 h. Acidic conditions were obligatory, since the enrichment culture was not able to produce methane or to grow at pH 7. Based on morphological characteristics, the dominant methanogenic species in the enrichment culture was a Methanosarcina.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalFEMS Microbiology Letters
Issue number1
StatePublished - May 1 1993


  • Acidophilic methanogenesis
  • Anaerobic degradation
  • Bioreactor
  • Methanol
  • Methanosarcina
  • Methylotrophic

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics


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