Accelerated communication a cloned NK2 receptor mediates phosphatidylinositol hydrolysis in a transfected murine fibrolast

Alden K. Henderson; Josephine Lai; Stephen H. Buck; Yutaka Fujiwara; Gurinder Singh; Mark S. Yamamura; Shigetada Nakanishi; William R. Roeske; Henry I. Yamamura

doi:10.1016/0024-3205(90)90329-P

Accelerated communication a cloned NK₂ receptor mediates phosphatidylinositol hydrolysis in a transfected murine fibrolast

Alden K. Henderson, Josephine Lai, Stephen H. Buck, Yutaka Fujiwara, Gurinder Singh, Mark S. Yamamura, Shigetada Nakanishi, William R. Roeske, Henry I. Yamamura

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17 Scopus citations

Abstract

Transfection of a bovine stomach cDNA encoding for an NK₂ receptor into murine fibroblasts produced a clone that exhibited specific binding of NKA, a selective NK₂ agonist. In these transfected cells, NKA mediated hydrolysis of phosphatidyl-inositol (PI) with an EC₅₀ value of 10 nM and an E_max value 7.2 times basal level. Dose response curves of IP₁ accumulation by two other neurokinin agonists demonstrated a rank order of potency of NKA>SP>>senktide. This suggests that the de novo receptor expressed in these transfected cells is functionally coupled to the PI hydrolysis pathway. Stable in vitro expression of NK₂ receptors in a eukaryotik cell line can provide a means of correlating the structurally defined receptor with its ligand binding properties and its functional coupling mechanisms.

Original language	English (US)
Pages (from-to)	PL7-PL12
Journal	Life Sciences
Volume	47
Issue number	3
DOIs	https://doi.org/10.1016/0024-3205(90)90329-P
State	Published - 1990

ASJC Scopus subject areas

General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry, Genetics and Molecular Biology

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@article{21d47c309ac44d7a9ba423401bfbb736,

title = "Accelerated communication a cloned NK2 receptor mediates phosphatidylinositol hydrolysis in a transfected murine fibrolast",

abstract = "Transfection of a bovine stomach cDNA encoding for an NK2 receptor into murine fibroblasts produced a clone that exhibited specific binding of NKA, a selective NK2 agonist. In these transfected cells, NKA mediated hydrolysis of phosphatidyl-inositol (PI) with an EC50 value of 10 nM and an Emax value 7.2 times basal level. Dose response curves of IP1 accumulation by two other neurokinin agonists demonstrated a rank order of potency of NKA>SP>>senktide. This suggests that the de novo receptor expressed in these transfected cells is functionally coupled to the PI hydrolysis pathway. Stable in vitro expression of NK2 receptors in a eukaryotik cell line can provide a means of correlating the structurally defined receptor with its ligand binding properties and its functional coupling mechanisms.",

author = "Henderson, {Alden K.} and Josephine Lai and Buck, {Stephen H.} and Yutaka Fujiwara and Gurinder Singh and Yamamura, {Mark S.} and Shigetada Nakanishi and Roeske, {William R.} and Yamamura, {Henry I.}",

note = "Funding Information: The authors thank Carol Haussler and Alfred Gallegos for preparing the tissue cultures and Dr. Lin Mei for guidance with the IP I accumulation assays. This work was partially supported by U.S. Public Health Service grant DK-36289, program project grant HL-20984, and a grant from Merrell Dow Research Institute.",

year = "1990",

doi = "10.1016/0024-3205(90)90329-P",

language = "English (US)",

volume = "47",

pages = "PL7--PL12",

journal = "Life Sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

number = "3",

}

TY - JOUR

T1 - Accelerated communication a cloned NK2 receptor mediates phosphatidylinositol hydrolysis in a transfected murine fibrolast

AU - Henderson, Alden K.

AU - Lai, Josephine

AU - Buck, Stephen H.

AU - Fujiwara, Yutaka

AU - Singh, Gurinder

AU - Yamamura, Mark S.

AU - Nakanishi, Shigetada

AU - Roeske, William R.

AU - Yamamura, Henry I.

N1 - Funding Information: The authors thank Carol Haussler and Alfred Gallegos for preparing the tissue cultures and Dr. Lin Mei for guidance with the IP I accumulation assays. This work was partially supported by U.S. Public Health Service grant DK-36289, program project grant HL-20984, and a grant from Merrell Dow Research Institute.

PY - 1990

Y1 - 1990

N2 - Transfection of a bovine stomach cDNA encoding for an NK2 receptor into murine fibroblasts produced a clone that exhibited specific binding of NKA, a selective NK2 agonist. In these transfected cells, NKA mediated hydrolysis of phosphatidyl-inositol (PI) with an EC50 value of 10 nM and an Emax value 7.2 times basal level. Dose response curves of IP1 accumulation by two other neurokinin agonists demonstrated a rank order of potency of NKA>SP>>senktide. This suggests that the de novo receptor expressed in these transfected cells is functionally coupled to the PI hydrolysis pathway. Stable in vitro expression of NK2 receptors in a eukaryotik cell line can provide a means of correlating the structurally defined receptor with its ligand binding properties and its functional coupling mechanisms.

AB - Transfection of a bovine stomach cDNA encoding for an NK2 receptor into murine fibroblasts produced a clone that exhibited specific binding of NKA, a selective NK2 agonist. In these transfected cells, NKA mediated hydrolysis of phosphatidyl-inositol (PI) with an EC50 value of 10 nM and an Emax value 7.2 times basal level. Dose response curves of IP1 accumulation by two other neurokinin agonists demonstrated a rank order of potency of NKA>SP>>senktide. This suggests that the de novo receptor expressed in these transfected cells is functionally coupled to the PI hydrolysis pathway. Stable in vitro expression of NK2 receptors in a eukaryotik cell line can provide a means of correlating the structurally defined receptor with its ligand binding properties and its functional coupling mechanisms.

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U2 - 10.1016/0024-3205(90)90329-P

DO - 10.1016/0024-3205(90)90329-P

M3 - Article

AN - SCOPUS:18744436966

SN - 0024-3205

VL - 47

SP - PL7-PL12

JO - Life Sciences

JF - Life Sciences

IS - 3

ER -

Accelerated communication a cloned NK₂ receptor mediates phosphatidylinositol hydrolysis in a transfected murine fibrolast

Abstract

ASJC Scopus subject areas

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