A yellow head virus gene probe: Nucleotide sequence and application for in situ hybridization

Kathy F.J. Tang, Donald V. Lightner

    Research output: Contribution to journalArticlepeer-review

    56 Scopus citations

    Abstract

    A portion of the genome of yellow head virus (YHV) of penaeid shrimp was cloned and the cDNA fragment (1161 bp) was designated clone 3-27. The fragment was labeled with digoxigenin and hybridized in situ to tissue sections of YHV-infected Penaeus vannamei. Positively reacting tissues included those of the lymphoid organ, cuticular epithelium, and gills. In addition, connective tissue of hepatopancreas, heart, antennal gland, hematopoietic organ, nerve tract, midgut cecum and muscle reacted to the probe. The probe was highly specific since it hybridized only to tissues from YHV-infected shrimp. It did not react to those of uninfected shrimp or shrimp infected with WSSV (white spot syndrome virus), IHHNV (infectious hypodermal and hematopoietic necrosis virus), or TSV (Taura syndrome virus). The clone was sequenced, and primers were synthesized for rapid detection of YHV in hemolymph using RT-PCR (reverse transcription-polymerase chain reaction). The strand that constituted the viral sequence in the cDNA was also determined via RT-PCR and in situ hybridization with a single-stranded RNA (ssRNA) probe.

    Original languageEnglish (US)
    Pages (from-to)165-173
    Number of pages9
    JournalDiseases of aquatic organisms
    Volume35
    Issue number3
    DOIs
    StatePublished - Feb 26 1999

    Keywords

    • In situ hybridization
    • Penaeid shrimp
    • RT-PCR
    • Yellow head virus

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Aquatic Science

    Fingerprint

    Dive into the research topics of 'A yellow head virus gene probe: Nucleotide sequence and application for in situ hybridization'. Together they form a unique fingerprint.

    Cite this