A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death

Jennifer L. Furman; Pui Wing Mok; Shengyi Shen; Cliff I. Stains; Indraneel Ghosh

doi:10.1039/c0cc02229b

A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death

Jennifer L. Furman
, Pui Wing Mok
, Shengyi Shen
, Cliff I. Stains
, Indraneel Ghosh

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Designed sensors comprising split-firefly luciferase conjugated to tandem poly(ADP-ribose) binding domains allow for the direct solution phase detection of picogram quantities of PAR and for monitoring temporal changes in poly(ADP-ribosyl)ation events in mammalian cells.

Original language	English (US)
Pages (from-to)	397-399
Number of pages	3
Journal	Chemical Communications
Volume	47
Issue number	1
DOIs	https://doi.org/10.1039/c0cc02229b
State	Published - Dec 6 2011

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Catalysis
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/c0cc02229b

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title = "A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death",

abstract = "Designed sensors comprising split-firefly luciferase conjugated to tandem poly(ADP-ribose) binding domains allow for the direct solution phase detection of picogram quantities of PAR and for monitoring temporal changes in poly(ADP-ribosyl)ation events in mammalian cells.",

author = "Furman, \{Jennifer L.\} and Mok, \{Pui Wing\} and Shengyi Shen and Stains, \{Cliff I.\} and Indraneel Ghosh",

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doi = "10.1039/c0cc02229b",

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AU - Furman, Jennifer L.

AU - Mok, Pui Wing

AU - Shen, Shengyi

AU - Stains, Cliff I.

AU - Ghosh, Indraneel

PY - 2011/12/6

Y1 - 2011/12/6

N2 - Designed sensors comprising split-firefly luciferase conjugated to tandem poly(ADP-ribose) binding domains allow for the direct solution phase detection of picogram quantities of PAR and for monitoring temporal changes in poly(ADP-ribosyl)ation events in mammalian cells.

AB - Designed sensors comprising split-firefly luciferase conjugated to tandem poly(ADP-ribose) binding domains allow for the direct solution phase detection of picogram quantities of PAR and for monitoring temporal changes in poly(ADP-ribosyl)ation events in mammalian cells.

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UR - https://www.scopus.com/pages/publications/78649842342#tab=citedBy

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AN - SCOPUS:78649842342

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EP - 399

JO - Chemical Communications

JF - Chemical Communications

IS - 1

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A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death

Abstract

ASJC Scopus subject areas

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